Human Gene TK1 (ENST00000301634.12_7) from GENCODE V47lift37
  Description: thymidine kinase 1, transcript variant 1 (from RefSeq NM_003258.5)
Gencode Transcript: ENST00000301634.12_7
Gencode Gene: ENSG00000167900.12_11
Transcript (Including UTRs)
   Position: hg19 chr17:76,170,172-76,183,133 Size: 12,962 Total Exon Count: 7 Strand: -
Coding Region
   Position: hg19 chr17:76,170,840-76,183,075 Size: 12,236 Coding Exon Count: 7 

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Gene AllelesRNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther Species
GO AnnotationsmRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr17:76,170,172-76,183,133)mRNA (may differ from genome)Protein (234 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
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HGNCMalacardsMGIOMIMPubMedReactome
UniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: KITH_HUMAN
DESCRIPTION: RecName: Full=Thymidine kinase, cytosolic; EC=2.7.1.21;
CATALYTIC ACTIVITY: ATP + thymidine = ADP + thymidine 5'- phosphate.
SUBUNIT: Homotetramer.
SUBCELLULAR LOCATION: Cytoplasm.
PTM: Phosphorylated on Ser-13 in mitosis.
MISCELLANEOUS: Two forms have been identified in animal cells, one in cytosol and one in mitochondria. Activity of the cytosolic enzyme is high in proliferating cells and peaks during the S-phase of the cell cycle; it is very low in resting cells.
SIMILARITY: Belongs to the thymidine kinase family.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: TK1
Diseases sorted by gene-association score: colorectal cancer 2 (4), lung cancer (2), chronic lymphocytic leukemia (2)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 45.85 RPKM in Cells - EBV-transformed lymphocytes
Total median expression: 191.41 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -18.1058-0.312 Picture PostScript Text
3' UTR -259.30668-0.388 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR001267 - Thymidine_kinase
IPR020633 - Thymidine_kinase_CS

Pfam Domains:
PF00265 - Thymidine kinase

SCOP Domains:
56784 - HAD-like
52540 - P-loop containing nucleoside triphosphate hydrolases
57716 - Glucocorticoid receptor-like (DNA-binding domain)

Protein Data Bank (PDB) 3-D Structure
MuPIT help
1W4R - X-ray MuPIT 1XBT - X-ray MuPIT 2ORV - X-ray MuPIT 2WVJ - X-ray MuPIT


ModBase Predicted Comparative 3D Structure on P04183
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details  Gene Details 
Gene SorterGene Sorter  Gene Sorter 
 RGDEnsembl WormBase 
      
      

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000166 nucleotide binding
GO:0004797 thymidine kinase activity
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0008270 zinc ion binding
GO:0016301 kinase activity
GO:0016740 transferase activity
GO:0019206 nucleoside kinase activity
GO:0042802 identical protein binding
GO:0046872 metal ion binding

Biological Process:
GO:0006139 nucleobase-containing compound metabolic process
GO:0006259 DNA metabolic process
GO:0009157 deoxyribonucleoside monophosphate biosynthetic process
GO:0009165 nucleotide biosynthetic process
GO:0016310 phosphorylation
GO:0043097 pyrimidine nucleoside salvage
GO:0046104 thymidine metabolic process
GO:0051289 protein homotetramerization
GO:0071897 DNA biosynthetic process

Cellular Component:
GO:0005737 cytoplasm
GO:0005829 cytosol


-  Descriptions from all associated GenBank mRNAs
  BC072024 - Homo sapiens cDNA clone IMAGE:6461728, **** WARNING: chimeric clone ****.
KJ892281 - Synthetic construct Homo sapiens clone ccsbBroadEn_01675 TK1 gene, encodes complete protein.
KJ897675 - Synthetic construct Homo sapiens clone ccsbBroadEn_07069 TK1 gene, encodes complete protein.
KJ905330 - Synthetic construct Homo sapiens clone ccsbBroadEn_14865 TK1 gene, encodes complete protein.
BC007872 - Homo sapiens thymidine kinase 1, soluble, mRNA (cDNA clone MGC:14441 IMAGE:4303880), complete cds.
BC007986 - Homo sapiens thymidine kinase 1, soluble, mRNA (cDNA clone MGC:3644 IMAGE:2966331), complete cds.
GQ891524 - Homo sapiens clone HEL-S-245 epididymis secretory sperm binding protein mRNA, complete cds.
BC006484 - Homo sapiens thymidine kinase 1, soluble, mRNA (cDNA clone MGC:4394 IMAGE:2905608), complete cds.
K02581 - Human thymidine kinase mRNA, complete cds.
JD087248 - Sequence 68272 from Patent EP1572962.
JD364054 - Sequence 345078 from Patent EP1572962.
JD287424 - Sequence 268448 from Patent EP1572962.
JD421364 - Sequence 402388 from Patent EP1572962.
JD194084 - Sequence 175108 from Patent EP1572962.
JD105475 - Sequence 86499 from Patent EP1572962.
JD467557 - Sequence 448581 from Patent EP1572962.
JD544557 - Sequence 525581 from Patent EP1572962.
JD279068 - Sequence 260092 from Patent EP1572962.
JD295057 - Sequence 276081 from Patent EP1572962.
JD320770 - Sequence 301794 from Patent EP1572962.
JD254282 - Sequence 235306 from Patent EP1572962.
JD446129 - Sequence 427153 from Patent EP1572962.
JD080032 - Sequence 61056 from Patent EP1572962.
JD371102 - Sequence 352126 from Patent EP1572962.
JD392669 - Sequence 373693 from Patent EP1572962.
JD260618 - Sequence 241642 from Patent EP1572962.
JD290684 - Sequence 271708 from Patent EP1572962.
JD021114 - Sequence 2138 from Patent EP1572962.
AK314950 - Homo sapiens cDNA, FLJ95862, highly similar to Homo sapiens thymidine kinase 1, soluble (TK1), mRNA.
AB451268 - Homo sapiens TK1 mRNA for thymidine kinase 1 soluble isoform, complete cds, clone: FLJ08092AAAN.
BT006941 - Homo sapiens thymidine kinase 1, soluble mRNA, complete cds.
AB451395 - Homo sapiens TK1 mRNA for thymidine kinase 1 soluble isoform, partial cds, clone: FLJ08092AAAF.
AB591021 - Synthetic construct DNA, clone: pFN21AE1723, Homo sapiens TK1 gene for thymidine kinase 1, soluble, without stop codon, in Flexi system.
JD024049 - Sequence 5073 from Patent EP1572962.
JD030634 - Sequence 11658 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  BioCyc Knowledge Library
PWY-7199 - pyrimidine deoxyribonucleosides salvage
PWY-7200 - superpathway of pyrimidine deoxyribonucleoside salvage

Reactome (by CSHL, EBI, and GO)

Protein P04183 (Reactome details) participates in the following event(s):

R-HSA-73632 thymidine + ATP => TMP (deoxythymidine 5'-monophosphate) + ADP [TK1]
R-HSA-539107 Activation of E2F1 target genes at G1/S
R-HSA-69205 G1/S-Specific Transcription
R-HSA-73614 Pyrimidine salvage
R-HSA-69206 G1/S Transition
R-HSA-8956321 Nucleotide salvage
R-HSA-453279 Mitotic G1-G1/S phases
R-HSA-15869 Metabolism of nucleotides
R-HSA-69278 Cell Cycle (Mitotic)
R-HSA-1430728 Metabolism
R-HSA-1640170 Cell Cycle

-  Other Names for This Gene
  Alternate Gene Symbols: B2RC58, ENST00000301634.1, ENST00000301634.10, ENST00000301634.11, ENST00000301634.2, ENST00000301634.3, ENST00000301634.4, ENST00000301634.5, ENST00000301634.6, ENST00000301634.7, ENST00000301634.8, ENST00000301634.9, KITH_HUMAN, NM_003258, P04183, Q969V0, Q9UMG9, TK1 , uc317mvr.1, uc317mvr.2
UCSC ID: ENST00000301634.12_7
RefSeq Accession: NM_003258.5
Protein: P04183 (aka KITH_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.