ID:TLK2_HUMAN DESCRIPTION: RecName: Full=Serine/threonine-protein kinase tousled-like 2; EC=2.7.11.1; AltName: Full=HsHPK; AltName: Full=PKU-alpha; AltName: Full=Tousled-like kinase 2; FUNCTION: Serine/threonine-protein kinase involved in the process of chromatin assembly and probably also DNA replication, transcription, repair, and chromosome segregation. Phosphorylates the chromatin assembly factors ASF1A AND ASF1B. Phosphorylation of ASF1A prevents its proteasome-mediated degradation, thereby enhancing chromatin assembly. Negative regulator of amino acid starvation-induced autophagy. CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein. COFACTOR: Magnesium. ENZYME REGULATION: Cell cycle-regulated, with maximal activity in the S-phase. Rapidly and transiently inhibited by phosphorylation following the generation of DNA double-stranded breaks during S- phase, probably by CHEK1, possibly at Ser-750. This inhibition is cell cycle checkpoint- and ATM-dependent. SUBUNIT: Monomer and heterodimer with TLK1. Interacts with ASF1A and ASF1B. Association with 14-3-3 proteins such as YWHAZ regulates subcellular location. May also interact with FEZ1/LZTS1 and FEZ2. INTERACTION: Q15306:IRF4; NbExp=2; IntAct=EBI-1047967, EBI-751345; Q92985:IRF7; NbExp=2; IntAct=EBI-1047967, EBI-968267; SUBCELLULAR LOCATION: Nucleus. Cytoplasm, perinuclear region. Cytoplasm, cytoskeleton. Note=Colocalizes with the cytoplasmic intermediate filament system during the G1 phase of the cell cycle. Present in the perinuclear region at S phase and in the nucleus at late G2. TISSUE SPECIFICITY: Ubiquitous. Detected in placenta, fetal liver, kidney, pancreas, heart and skeletal muscle. Highly expressed in testis. Detected in spleen, thymus, colon, ovary, small intestine, prostate and peripheral blood leukocytes. PTM: Phosphorylated at Ser-750, probably by CHEK1. SIMILARITY: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. SIMILARITY: Contains 1 protein kinase domain. SEQUENCE CAUTION: Sequence=AAF03095.1; Type=Erroneous initiation; Sequence=AAH44925.2; Type=Erroneous initiation; Sequence=BAA20561.1; Type=Erroneous initiation;
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
Pfam Domains: PF00069 - Protein kinase domain PF07714 - Protein tyrosine and serine/threonine kinase
SCOP Domains: 158682 - TerB-like 56112 - Protein kinase-like (PK-like)
ModBase Predicted Comparative 3D Structure on Q86UE8
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
US Server
Sequence and Links to Tools and Databases 
Common Gene Haplotype Alleles 
