Human Gene TNFRSF10B (ENST00000276431.9_8) from GENCODE V47lift37
  Description: TNF receptor superfamily member 10b, transcript variant 1 (from RefSeq NM_003842.5)
Gencode Transcript: ENST00000276431.9_8
Gencode Gene: ENSG00000120889.13_10
Transcript (Including UTRs)
   Position: hg19 chr8:22,877,646-22,926,544 Size: 48,899 Total Exon Count: 9 Strand: -
Coding Region
   Position: hg19 chr8:22,880,184-22,926,407 Size: 46,224 Coding Exon Count: 9 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
Gene AllelesRNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther Species
GO AnnotationsmRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr8:22,877,646-22,926,544)mRNA (may differ from genome)Protein (440 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGeneCardsHGNC
MalacardsMGIOMIMPubMedReactomeUniProtKB
WikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: TR10B_HUMAN
DESCRIPTION: RecName: Full=Tumor necrosis factor receptor superfamily member 10B; AltName: Full=Death receptor 5; AltName: Full=TNF-related apoptosis-inducing ligand receptor 2; Short=TRAIL receptor 2; Short=TRAIL-R2; AltName: CD_antigen=CD262; Flags: Precursor;
FUNCTION: Receptor for the cytotoxic ligand TNFSF10/TRAIL. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Promotes the activation of NF- kappa-B. Essential for ER stress-induced apoptosis.
SUBUNIT: Homotrimer. Can interact with TRADD and RIPK1.
INTERACTION: P50591:TNFSF10; NbExp=23; IntAct=EBI-518882, EBI-495373;
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
TISSUE SPECIFICITY: Widely expressed in adult and fetal tissues; very highly expressed in tumor cell lines such as HeLaS3, K-562, HL-60, SW480, A-549 and G-361; highly expressed in heart, peripheral blood lymphocytes, liver, pancreas, spleen, thymus, prostate, ovary, uterus, placenta, testis, esophagus, stomach and throughout the intestinal tract; not detectable in brain.
INDUCTION: By ER stress. Regulated by p53/TP53.
DISEASE: Defects in TNFRSF10B may be a cause of head and neck squamous cell carcinomas (HNSCC) [MIM:275355]; also known as squamous cell carcinoma of the head and neck.
SIMILARITY: Contains 1 death domain.
SIMILARITY: Contains 3 TNFR-Cys repeats.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: TNFRSF10B
Diseases sorted by gene-association score: squamous cell carcinoma, head and neck* (537), diffuse infiltrative lymphocytosis syndrome (9), malignant glioma (8), iridocyclitis (7), diffuse scleroderma (7), oral cavity cancer (6), toxic diffuse goiter (4), palindromic rheumatism (4), la crosse encephalitis (4), geographic tongue (4), lung cancer (3), colorectal cancer (3), hepatocellular carcinoma (2), renal cell carcinoma (2), ovarian cancer, somatic (2), prostate cancer (2)
* = Manually curated disease association

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 41.58 RPKM in Cells - EBV-transformed lymphocytes
Total median expression: 423.78 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -29.50137-0.215 Picture PostScript Text
3' UTR -811.002538-0.320 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR000488 - Death
IPR011029 - DEATH-like
IPR001368 - TNFR/NGFR_Cys_rich_reg
IPR020465 - TNFR_10

Pfam Domains:
PF00020 - TNFR/NGFR cysteine-rich region
PF00531 - Death domain

SCOP Domains:
47986 - DEATH domain
57586 - TNF receptor-like
57184 - Growth factor receptor domain

Protein Data Bank (PDB) 3-D Structure
MuPIT help
1D0G - X-ray MuPIT 1D4V - X-ray MuPIT 1DU3 - X-ray MuPIT 1ZA3 - X-ray 2H9G - X-ray MuPIT


ModBase Predicted Comparative 3D Structure on O14763
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
Gene SorterGene Sorter    
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0005031 tumor necrosis factor-activated receptor activity
GO:0005515 protein binding
GO:0038023 signaling receptor activity
GO:0045569 TRAIL binding

Biological Process:
GO:0006915 apoptotic process
GO:0006919 activation of cysteine-type endopeptidase activity involved in apoptotic process
GO:0006954 inflammatory response
GO:0006955 immune response
GO:0007165 signal transduction
GO:0007166 cell surface receptor signaling pathway
GO:0007250 activation of NF-kappaB-inducing kinase activity
GO:0007275 multicellular organism development
GO:0008625 extrinsic apoptotic signaling pathway via death domain receptors
GO:0032496 response to lipopolysaccharide
GO:0033209 tumor necrosis factor-mediated signaling pathway
GO:0034976 response to endoplasmic reticulum stress
GO:0042127 regulation of cell proliferation
GO:0042981 regulation of apoptotic process
GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB signaling
GO:0043154 negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
GO:0050900 leukocyte migration
GO:0070059 intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress
GO:0071260 cellular response to mechanical stimulus
GO:1902041 regulation of extrinsic apoptotic signaling pathway via death domain receptors
GO:1902042 negative regulation of extrinsic apoptotic signaling pathway via death domain receptors

Cellular Component:
GO:0005622 intracellular
GO:0005886 plasma membrane
GO:0005887 integral component of plasma membrane
GO:0009986 cell surface
GO:0016020 membrane
GO:0016021 integral component of membrane


-  Descriptions from all associated GenBank mRNAs
  AF016266 - Homo sapiens TRAIL receptor 2 mRNA, complete cds.
BX537617 - Homo sapiens mRNA; cDNA DKFZp686A24188 (from clone DKFZp686A24188).
BX538179 - Homo sapiens mRNA; cDNA DKFZp686B23188 (from clone DKFZp686B23188).
BX538104 - Homo sapiens mRNA; cDNA DKFZp686I01145 (from clone DKFZp686I01145).
JD326306 - Sequence 307330 from Patent EP1572962.
JD504246 - Sequence 485270 from Patent EP1572962.
JD297281 - Sequence 278305 from Patent EP1572962.
JD234814 - Sequence 215838 from Patent EP1572962.
JD495246 - Sequence 476270 from Patent EP1572962.
JD090296 - Sequence 71320 from Patent EP1572962.
JD107505 - Sequence 88529 from Patent EP1572962.
JD505736 - Sequence 486760 from Patent EP1572962.
JD356304 - Sequence 337328 from Patent EP1572962.
JD560776 - Sequence 541800 from Patent EP1572962.
JD417137 - Sequence 398161 from Patent EP1572962.
JD398261 - Sequence 379285 from Patent EP1572962.
JD070337 - Sequence 51361 from Patent EP1572962.
JD262164 - Sequence 243188 from Patent EP1572962.
JD087155 - Sequence 68179 from Patent EP1572962.
JD523013 - Sequence 504037 from Patent EP1572962.
JD418660 - Sequence 399684 from Patent EP1572962.
JD418781 - Sequence 399805 from Patent EP1572962.
JD325640 - Sequence 306664 from Patent EP1572962.
JD367105 - Sequence 348129 from Patent EP1572962.
JD413622 - Sequence 394646 from Patent EP1572962.
JD413623 - Sequence 394647 from Patent EP1572962.
JD455707 - Sequence 436731 from Patent EP1572962.
JD414335 - Sequence 395359 from Patent EP1572962.
JD187867 - Sequence 168891 from Patent EP1572962.
JD402809 - Sequence 383833 from Patent EP1572962.
JD494717 - Sequence 475741 from Patent EP1572962.
JD386032 - Sequence 367056 from Patent EP1572962.
JD223013 - Sequence 204037 from Patent EP1572962.
JD466277 - Sequence 447301 from Patent EP1572962.
JD432327 - Sequence 413351 from Patent EP1572962.
JD486265 - Sequence 467289 from Patent EP1572962.
JD316985 - Sequence 298009 from Patent EP1572962.
JD147045 - Sequence 128069 from Patent EP1572962.
JD110408 - Sequence 91432 from Patent EP1572962.
JD348768 - Sequence 329792 from Patent EP1572962.
JD161983 - Sequence 143007 from Patent EP1572962.
JD280074 - Sequence 261098 from Patent EP1572962.
JD391132 - Sequence 372156 from Patent EP1572962.
JD051962 - Sequence 32986 from Patent EP1572962.
JD331432 - Sequence 312456 from Patent EP1572962.
JD507535 - Sequence 488559 from Patent EP1572962.
JD157784 - Sequence 138808 from Patent EP1572962.
JD078106 - Sequence 59130 from Patent EP1572962.
JD334956 - Sequence 315980 from Patent EP1572962.
JD415379 - Sequence 396403 from Patent EP1572962.
JD508473 - Sequence 489497 from Patent EP1572962.
JD560911 - Sequence 541935 from Patent EP1572962.
JD450586 - Sequence 431610 from Patent EP1572962.
JD454252 - Sequence 435276 from Patent EP1572962.
JD408807 - Sequence 389831 from Patent EP1572962.
JD220323 - Sequence 201347 from Patent EP1572962.
JD287562 - Sequence 268586 from Patent EP1572962.
AK291216 - Homo sapiens cDNA FLJ76289 complete cds, highly similar to Homo sapiens tumor necrosis factor receptor superfamily, member 10b (TNFRSF10B), transcript variant 1, mRNA.
JD113945 - Sequence 94969 from Patent EP1572962.
AF016268 - Homo sapiens death receptor 5 (DR5) mRNA, complete cds.
AK296128 - Homo sapiens cDNA FLJ59908 complete cds, highly similar to Tumor necrosis factor receptor superfamily member 10B precursor.
AF192548 - Homo sapiens tumor necrosis factor receptor-like protein ZTNFR9 (ZTNFR9) mRNA, complete cds.
AF012535 - Homo sapiens death receptor 5 (DR5) mRNA, complete cds.
AY358277 - Homo sapiens clone DNA27868 TNFRSF10B/DR5 (UNQ160) mRNA, complete cds.
AF016849 - Homo sapiens apoptosis inducing receptor TRAIL-R2 (TRAILR2) mRNA, complete cds.
AF153687 - Homo sapiens Fas-like protein precusor mRNA, complete cds.
BC001281 - Homo sapiens tumor necrosis factor receptor superfamily, member 10b, mRNA (cDNA clone MGC:5144 IMAGE:3458466), complete cds.
AK298602 - Homo sapiens cDNA FLJ52718 complete cds, highly similar to Homo sapiens tumor necrosis factor receptor superfamily, member 10b, transcript variant 2, mRNA.
JD539146 - Sequence 520170 from Patent EP1572962.
JD434542 - Sequence 415566 from Patent EP1572962.
JD415679 - Sequence 396703 from Patent EP1572962.
JD281714 - Sequence 262738 from Patent EP1572962.
JD259816 - Sequence 240840 from Patent EP1572962.
JD048862 - Sequence 29886 from Patent EP1572962.
AB451272 - Homo sapiens TNFRSF10B mRNA for tumor necrosis factor receptor superfamily member 10B precursor, complete cds, clone: FLJ08099AAAN.
AF012628 - Homo sapiens death domain containing receptor for TRAIL/Apo-2L (DR5) mRNA, complete cds.
AF018657 - Homo sapiens apoptosis inducing protein (TRICK2A) mRNA, complete cds.
AF018658 - Homo sapiens apoptosis inducing protein (TRICK2B) mRNA, complete cds.
AF020501 - Homo sapiens cytotoxic TRAIL receptor-2 (DR5) mRNA, complete cds.
AF022386 - Homo sapiens p53-regulated DNA damage-inducible cell death receptor (killer) mRNA, complete cds.
CR541898 - Homo sapiens full open reading frame cDNA clone RZPDo834D1033D for gene TNFRSF10B, tumor necrosis factor receptor superfamily, member 10b; complete cds, incl. stopcodon.
FJ200482 - Homo sapiens TNF receptor superfamily member 10b variant mRNA, complete cds.
KJ897910 - Synthetic construct Homo sapiens clone ccsbBroadEn_07304 TNFRSF10B gene, encodes complete protein.
AB451399 - Homo sapiens TNFRSF10B mRNA for tumor necrosis factor receptor superfamily member 10B precursor, partial cds, clone: FLJ08099AAAF.
AB385139 - Synthetic construct DNA, clone: pF1KB5639, Homo sapiens TNFRSF10B gene for tumor necrosis factor receptor superfamily member 10B precursor, complete cds, without stop codon, in Flexi system.
DQ596320 - Homo sapiens piRNA piR-34386, complete sequence.
JD386519 - Sequence 367543 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein O14763 (Reactome details) participates in the following event(s):

R-HSA-75238 TRAIL binds TRAIL receptors
R-HSA-5635741 TRAIL receptor-4 binds TRAIL receptor-1 or TRAIL receptor-2
R-HSA-141139 Trimerization of TRAIL:TRAIL receptor complex
R-HSA-75187 TRAIL:TRAIL receptor trimers bind FADD
R-HSA-75146 TRAIL:TRAIL Receptor Trimer:FADD complex binds procaspase-8
R-HSA-141316 TRAIL:TRAIL-Receptor2 Trimer:FADD complex binds procaspase-10
R-HSA-141156 TRAIL-mediated dimerization of procaspase-8
R-HSA-3371359 FLIP(L) and procaspase-8 form heterodimer in TRAIL signaling
R-HSA-3465429 FLIP(S) and procaspase-8 form heterodimer
R-HSA-5675456 FLIP(L) and procaspase-8 form heterodimer
R-HSA-75158 TRAIL signaling
R-HSA-202733 Cell surface interactions at the vascular wall
R-HSA-6803211 TP53 Regulates Transcription of Death Receptors and Ligands
R-HSA-73887 Death Receptor Signalling
R-HSA-109582 Hemostasis
R-HSA-5633008 TP53 Regulates Transcription of Cell Death Genes
R-HSA-162582 Signal Transduction
R-HSA-3700989 Transcriptional Regulation by TP53
R-HSA-212436 Generic Transcription Pathway
R-HSA-69416 Dimerization of procaspase-8
R-HSA-3371378 Regulation by c-FLIP
R-HSA-140534 Ligand-dependent caspase activation
R-HSA-73857 RNA Polymerase II Transcription
R-HSA-5218900 CASP8 activity is inhibited
R-HSA-5357769 Caspase activation via extrinsic apoptotic signalling pathway
R-HSA-74160 Gene expression (Transcription)
R-HSA-5675482 Regulation of necroptotic cell death
R-HSA-109581 Apoptosis
R-HSA-5213460 RIPK1-mediated regulated necrosis
R-HSA-5357801 Programmed Cell Death
R-HSA-5218859 Regulated Necrosis

-  Other Names for This Gene
  Alternate Gene Symbols: DR5, ENST00000276431.1, ENST00000276431.2, ENST00000276431.3, ENST00000276431.4, ENST00000276431.5, ENST00000276431.6, ENST00000276431.7, ENST00000276431.8, KILLER, NM_003842, O14720, O14763, O15508, O15517, O15531, Q6UXM8, Q7Z360, Q9BVE0, TR10B_HUMAN, TRAILR2, TRICK2, uc317jkl.1, uc317jkl.2, UNQ160/PRO186, ZTNFR9
UCSC ID: ENST00000276431.9_8
RefSeq Accession: NM_003842.5
Protein: O14763 (aka TR10B_HUMAN or T10B_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.