Human Gene TP53RK (ENST00000372114.4_9) from GENCODE V47lift37
  Description: TP53 regulating kinase (from RefSeq NM_033550.4)
Gencode Transcript: ENST00000372114.4_9
Gencode Gene: ENSG00000172315.6_11
Transcript (Including UTRs)
   Position: hg19 chr20:45,313,004-45,318,083 Size: 5,080 Total Exon Count: 2 Strand: -
Coding Region
   Position: hg19 chr20:45,315,392-45,318,053 Size: 2,662 Coding Exon Count: 2 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
Gene AllelesRNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther Species
GO AnnotationsmRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr20:45,313,004-45,318,083)mRNA (may differ from genome)Protein (253 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
HGNCMalacardsMGIOMIMPubMedReactome
UniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: PRPK_HUMAN
DESCRIPTION: RecName: Full=TP53-regulating kinase; EC=2.7.11.1; AltName: Full=Nori-2; AltName: Full=p53-related protein kinase;
FUNCTION: Protein kinase that phosphorylates 'Ser-15' of p53/TP53 protein and may therefore participate in its activation.
CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.
SUBUNIT: Interacts with TPRKB.
SUBCELLULAR LOCATION: Nucleus.
TISSUE SPECIFICITY: Highly expressed in testis. Weakly expressed in heart kidney and spleen.
SIMILARITY: Belongs to the protein kinase superfamily.
SIMILARITY: Contains 1 protein kinase domain.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: TP53RK
Diseases sorted by gene-association score: narcolepsy (2)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 10.42 RPKM in Testis
Total median expression: 213.17 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -9.9030-0.330 Picture PostScript Text
3' UTR -648.202388-0.271 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR022495 - Kae1-assoc_kinase_Bud32
IPR011009 - Kinase-like_dom
IPR010440 - LipoPS_kinase
IPR000719 - Prot_kinase_cat_dom
IPR008266 - Tyr_kinase_AS

Pfam Domains:
PF00069 - Protein kinase domain
PF01163 - RIO1 family
PF06293 - Lipopolysaccharide kinase (Kdo/WaaP) family
PF07714 - Protein tyrosine and serine/threonine kinase

SCOP Domains:
56112 - Protein kinase-like (PK-like)

ModBase Predicted Comparative 3D Structure on Q96S44
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologGenome Browser
   Gene DetailsGene DetailsGene Details
   Gene SorterGene SorterGene Sorter
  Ensembl WormBaseSGD
     Protein Sequence
     Alignment

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000166 nucleotide binding
GO:0002039 p53 binding
GO:0004672 protein kinase activity
GO:0004674 protein serine/threonine kinase activity
GO:0005515 protein binding
GO:0005524 ATP binding
GO:0016301 kinase activity
GO:0016740 transferase activity
GO:0016773 phosphotransferase activity, alcohol group as acceptor
GO:0016787 hydrolase activity

Biological Process:
GO:0006468 protein phosphorylation
GO:0008033 tRNA processing
GO:0016310 phosphorylation
GO:0070525 tRNA threonylcarbamoyladenosine metabolic process
GO:1901796 regulation of signal transduction by p53 class mediator

Cellular Component:
GO:0000408 EKC/KEOPS complex
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0016020 membrane


-  Descriptions from all associated GenBank mRNAs
  AK096658 - Homo sapiens cDNA FLJ39339 fis, clone OCBBF2018012, highly similar to Homo sapiens sodium-dependent high-affinity dicarboxylate transporter (NADC3) mRNA.
BC010637 - Homo sapiens TP53 regulating kinase, mRNA (cDNA clone IMAGE:3854842), partial cds.
BC035461 - Homo sapiens TP53 regulating kinase, mRNA (cDNA clone IMAGE:3854798).
AK096502 - Homo sapiens cDNA FLJ39183 fis, clone OCBBF2004273, highly similar to TP53-regulating kinase (EC 2.7.11.1).
BC019621 - Homo sapiens cDNA clone IMAGE:3461030, containing frame-shift errors.
AB065434 - Homo sapiens prpk mRNA for PRPK mutant1, complete cds.
JD486410 - Sequence 467434 from Patent EP1572962.
JD500485 - Sequence 481509 from Patent EP1572962.
JD298871 - Sequence 279895 from Patent EP1572962.
JD220531 - Sequence 201555 from Patent EP1572962.
JD551133 - Sequence 532157 from Patent EP1572962.
JD436194 - Sequence 417218 from Patent EP1572962.
JD181661 - Sequence 162685 from Patent EP1572962.
JD348838 - Sequence 329862 from Patent EP1572962.
BC066309 - Homo sapiens TP53 regulating kinase, mRNA (cDNA clone MGC:87135 IMAGE:5277911), complete cds.
JD318310 - Sequence 299334 from Patent EP1572962.
JD103003 - Sequence 84027 from Patent EP1572962.
JD546862 - Sequence 527886 from Patent EP1572962.
BC009727 - Homo sapiens TP53 regulating kinase, mRNA (cDNA clone MGC:9600 IMAGE:3899629), complete cds.
AB017505 - Homo sapiens mRNA for Nori-2p, complete cds.
JD354515 - Sequence 335539 from Patent EP1572962.
JD232274 - Sequence 213298 from Patent EP1572962.
AK315182 - Homo sapiens cDNA, FLJ96161.
KJ895239 - Synthetic construct Homo sapiens clone ccsbBroadEn_04633 TP53RK gene, encodes complete protein.
KJ905594 - Synthetic construct Homo sapiens clone ccsbBroadEn_15220 TP53RK gene, encodes complete protein.
AB587589 - Synthetic construct DNA, clone: pF1KB8913, Homo sapiens TP53RK gene for TP53 regulating kinase, without stop codon, in Flexi system.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q96S44 (Reactome details) participates in the following event(s):

R-HSA-6805479 TP53RK phosphorylates TP53
R-HSA-6784494 EKC complex threonylcarbamoylates A37 of tRNAs
R-HSA-6804756 Regulation of TP53 Activity through Phosphorylation
R-HSA-6782315 tRNA modification in the nucleus and cytosol
R-HSA-5633007 Regulation of TP53 Activity
R-HSA-72306 tRNA processing
R-HSA-3700989 Transcriptional Regulation by TP53
R-HSA-8953854 Metabolism of RNA
R-HSA-212436 Generic Transcription Pathway
R-HSA-73857 RNA Polymerase II Transcription
R-HSA-74160 Gene expression (Transcription)

-  Other Names for This Gene
  Alternate Gene Symbols: B3KU44, C20orf64, ENST00000372114.1, ENST00000372114.2, ENST00000372114.3, NM_033550, PRPK , PRPK_HUMAN, Q3T977, Q5JZ01, Q6NZ60, Q96FM7, Q96S44, Q9NQE6, TP53RK , uc318jqy.1, uc318jqy.2
UCSC ID: ENST00000372114.4_9
RefSeq Accession: NM_033550.4
Protein: Q96S44 (aka PRPK_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.