Human Gene TRADD (ENST00000345057.9_7) from GENCODE V47lift37
  Description: TNFRSF1A associated via death domain, transcript variant 1 (from RefSeq NM_003789.4)
Gencode Transcript: ENST00000345057.9_7
Gencode Gene: ENSG00000102871.16_9
Transcript (Including UTRs)
   Position: hg19 chr16:67,188,088-67,193,812 Size: 5,725 Total Exon Count: 5 Strand: -
Coding Region
   Position: hg19 chr16:67,188,552-67,190,563 Size: 2,012 Coding Exon Count: 4 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr16:67,188,088-67,193,812)mRNA (may differ from genome)Protein (312 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
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-  Comments and Description Text from UniProtKB
  ID: TRADD_HUMAN
DESCRIPTION: RecName: Full=Tumor necrosis factor receptor type 1-associated DEATH domain protein; Short=TNFR1-associated DEATH domain protein; AltName: Full=TNFRSF1A-associated via death domain;
FUNCTION: The nuclear form acts as a tumor suppressor by preventing ubiquitination and degradation of isoform p19ARF/ARF of CDKN2A by TRIP12: acts by interacting with TRIP12, leading to disrupt interaction between TRIP12 and isoform p19ARF/ARF of CDKN2A (By similarity). Adapter molecule for TNFRSF1A/TNFR1 that specifically associates with the cytoplasmic domain of activated TNFRSF1A/TNFR1 mediating its interaction with FADD. Overexpression of TRADD leads to two major TNF-induced responses, apoptosis and activation of NF-kappa-B.
SUBUNIT: Interacts with TRIP12 (By similarity). Heterodimer with TNFRSF1A/TNFR1. Interacts with DAB2IP, FADD, HIPK2, KRT14, KRT16, KRT17, KRT18, RIPK1, SQSTM1, TRAF1, TRAF2 and TRPC4AP.
INTERACTION: Self; NbExp=3; IntAct=EBI-359215, EBI-359215; P05783:KRT18; NbExp=11; IntAct=EBI-359215, EBI-297888; P19438:TNFRSF1A; NbExp=10; IntAct=EBI-359215, EBI-299451;
SUBCELLULAR LOCATION: Nucleus (By similarity). Cytoplasm (By similarity). Cytoplasm, cytoskeleton. Note=Shuttles between the cytoplasm and the nucleus (By similarity).
TISSUE SPECIFICITY: Found in all examined tissues.
DOMAIN: Requires the intact death domain to associate with TNFRSF1A/TNFR1.
SIMILARITY: Contains 1 death domain.
SEQUENCE CAUTION: Sequence=AAA98482.1; Type=Erroneous initiation;
WEB RESOURCE: Name=SeattleSNPs; URL="http://pga.gs.washington.edu/data/tradd/";

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 24.60 RPKM in Spleen
Total median expression: 519.30 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -35.9080-0.449 Picture PostScript Text
3' UTR -166.30464-0.358 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR000488 - Death
IPR011029 - DEATH-like
IPR009095 - TRADD_N

Pfam Domains:
PF00531 - Death domain
PF09034 - TRADD, N-terminal domain

SCOP Domains:
47986 - DEATH domain
55044 - TRADD, N-terminal domain

Protein Data Bank (PDB) 3-D Structure
MuPIT help
1F2H - NMR MuPIT 1F3V - X-ray MuPIT


ModBase Predicted Comparative 3D Structure on Q15628
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0005164 tumor necrosis factor receptor binding
GO:0005515 protein binding
GO:0019900 kinase binding
GO:0042802 identical protein binding
GO:0044877 macromolecular complex binding
GO:0060090 binding, bridging
GO:0070513 death domain binding

Biological Process:
GO:0006915 apoptotic process
GO:0006919 activation of cysteine-type endopeptidase activity involved in apoptotic process
GO:0007165 signal transduction
GO:0007249 I-kappaB kinase/NF-kappaB signaling
GO:0008625 extrinsic apoptotic signaling pathway via death domain receptors
GO:0010803 regulation of tumor necrosis factor-mediated signaling pathway
GO:0033209 tumor necrosis factor-mediated signaling pathway
GO:0043065 positive regulation of apoptotic process
GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB signaling
GO:0051092 positive regulation of NF-kappaB transcription factor activity
GO:0051291 protein heterooligomerization
GO:0051798 positive regulation of hair follicle development
GO:0071550 death-inducing signaling complex assembly
GO:0097191 extrinsic apoptotic signaling pathway
GO:1902041 regulation of extrinsic apoptotic signaling pathway via death domain receptors
GO:1902042 negative regulation of extrinsic apoptotic signaling pathway via death domain receptors

Cellular Component:
GO:0005634 nucleus
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0005856 cytoskeleton
GO:0005886 plasma membrane
GO:0031264 death-inducing signaling complex
GO:0032991 macromolecular complex
GO:0043235 receptor complex
GO:0045121 membrane raft


-  Descriptions from all associated GenBank mRNAs
  AK090673 - Homo sapiens cDNA FLJ33354 fis, clone BRACE2005124, highly similar to Tumor necrosis factor receptor type1-associated DEATH domain protein.
BC004491 - Homo sapiens TNFRSF1A-associated via death domain, mRNA (cDNA clone MGC:11078 IMAGE:3689007), complete cds.
L41690 - Homo sapiens TNF receptor-1 associated protein (TRADD) mRNA, 3' end of cds.
JD255941 - Sequence 236965 from Patent EP1572962.
JD295087 - Sequence 276111 from Patent EP1572962.
JD436653 - Sequence 417677 from Patent EP1572962.
JD193009 - Sequence 174033 from Patent EP1572962.
JD104797 - Sequence 85821 from Patent EP1572962.
JD565426 - Sequence 546450 from Patent EP1572962.
JD250559 - Sequence 231583 from Patent EP1572962.
JD493500 - Sequence 474524 from Patent EP1572962.
BT006934 - Homo sapiens TNFRSF1A-associated via death domain mRNA, complete cds.
DQ893419 - Synthetic construct clone IMAGE:100006049; FLH199504.01X; RZPDo839E1282D TNFRSF1A-associated via death domain (TRADD) gene, encodes complete protein.
AK315654 - Homo sapiens cDNA, FLJ96745, Homo sapiens TNFRSF1A-associated via death domain (TRADD),transcript variant 1, mRNA.
DQ896738 - Synthetic construct Homo sapiens clone IMAGE:100011198; FLH199410.01L; RZPDo839E1281D TNFRSF1A-associated via death domain (TRADD) gene, encodes complete protein.
AB590160 - Synthetic construct DNA, clone: pFN21AE1362, Homo sapiens TRADD gene for TNFRSF1A-associated via death domain, without stop codon, in Flexi system.
JD271289 - Sequence 252313 from Patent EP1572962.
JD458507 - Sequence 439531 from Patent EP1572962.
AK301593 - Homo sapiens cDNA FLJ54928 complete cds, weakly similar to Tumor necrosis factor receptor type1-associated DEATH domain protein.
JD203690 - Sequence 184714 from Patent EP1572962.
LF213561 - JP 2014500723-A/21064: Polycomb-Associated Non-Coding RNAs.
MA449138 - JP 2018138019-A/21064: Polycomb-Associated Non-Coding RNAs.

-  Biochemical and Signaling Pathways
  BioCarta from NCI Cancer Genome Anatomy Project
h_HivnefPathway - HIV-I Nef: negative effector of Fas and TNF
h_deathPathway - Induction of apoptosis through DR3 and DR4/5 Death Receptors
h_ceramidePathway - Ceramide Signaling Pathway
h_tnfr1Pathway - TNFR1 Signaling Pathway
h_mapkPathway - MAPKinase Signaling Pathway
h_nfkbPathway - NF-kB Signaling Pathway
h_RELAPathway - Acetylation and Deacetylation of RelA in The Nucleus
h_p38mapkPathway - p38 MAPK Signaling Pathway
h_stressPathway - TNF/Stress Related Signaling
h_soddPathway - SODD/TNFR1 Signaling Pathway

Reactome (by CSHL, EBI, and GO)

Protein Q15628 (Reactome details) participates in the following event(s):

R-HSA-83656 TNF:TNFR1 binds TRADD, TRAF2 and RIPK1
R-HSA-5634221 TRAF1 binds TRAF2 within TNFR1 signaling complex
R-HSA-140978 TRADD:TRAF2:RIP1 complex binds FADD
R-HSA-83582 TRADD:TRAF2:RIP1 complex dissociates from the TNF-alpha:TNF-R1 complex.
R-HSA-5357780 TNF:TNFR1:TRADD:TRAF2:RIPK1 recruits BIRC2/3 (cIAP1/2)
R-HSA-5357860 TNF:TNFR1:TRADD:TRAF2:K63polyUb-RIPK1:BIRC2/3 recruits TAK1 complex
R-HSA-5357904 TNF:TNFR1:TRADD:TRAF2:RIPK1 binds LUBAC
R-HSA-5357928 CLIP3 and CYLD bind TNF signaling complex
R-HSA-5693055 TAX1BP1:TNFAIP3(A20) binds RIPK1-containing complexes
R-HSA-5357757 BIRC(cIAP1/2) ubiquitinates RIPK1
R-HSA-75240 TRADD:TRAF2:RIP1:FADD complex binds Pro-Caspase 8
R-HSA-5693108 TNFAIP3 (A20) ubiquitinates RIPK1 with K48-linked Ub chains
R-HSA-5357776 TNF:TNFR1:TRADD:TRAF2:K63polyUb-RIPK1:BIRC2,3:LUBAC recruits IKKA:IKBKB:IKBKG
R-HSA-141159 TNF-mediated dimerization of procaspase-8
R-HSA-3371360 FLIP(L) and procaspase-8 form heterodimer in TNF signaling
R-HSA-3465429 FLIP(S) and procaspase-8 form heterodimer
R-HSA-5675456 FLIP(L) and procaspase-8 form heterodimer
R-HSA-75893 TNF signaling
R-HSA-5357905 Regulation of TNFR1 signaling
R-HSA-5357786 TNFR1-induced proapoptotic signaling
R-HSA-5675482 Regulation of necroptotic cell death
R-HSA-5357956 TNFR1-induced NFkappaB signaling pathway
R-HSA-73887 Death Receptor Signalling
R-HSA-5213460 RIPK1-mediated regulated necrosis
R-HSA-162582 Signal Transduction
R-HSA-69416 Dimerization of procaspase-8
R-HSA-3371378 Regulation by c-FLIP
R-HSA-140534 Ligand-dependent caspase activation
R-HSA-5218859 Regulated Necrosis
R-HSA-5218900 CASP8 activity is inhibited
R-HSA-5357769 Caspase activation via extrinsic apoptotic signalling pathway
R-HSA-5357801 Programmed Cell Death
R-HSA-109581 Apoptosis

-  Other Names for This Gene
  Alternate Gene Symbols: B2RDS3, B3KQZ9, ENST00000345057.1, ENST00000345057.2, ENST00000345057.3, ENST00000345057.4, ENST00000345057.5, ENST00000345057.6, ENST00000345057.7, ENST00000345057.8, NM_003789, Q15628, Q52NZ1, TRADD , TRADD_HUMAN, uc317xdw.1, uc317xdw.2
UCSC ID: ENST00000345057.9_7
RefSeq Accession: NM_003789.4
Protein: Q15628 (aka TRADD_HUMAN or TRAD_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.