Human Gene TRMT61B (ENST00000306108.10_7) from GENCODE V47lift37
  Description: tRNA methyltransferase 61B (from RefSeq NM_017910.4)
Gencode Transcript: ENST00000306108.10_7
Gencode Gene: ENSG00000171103.11_10
Transcript (Including UTRs)
   Position: hg19 chr2:29,072,687-29,093,175 Size: 20,489 Total Exon Count: 7 Strand: -
Coding Region
   Position: hg19 chr2:29,073,065-29,093,143 Size: 20,079 Coding Exon Count: 7 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr2:29,072,687-29,093,175)mRNA (may differ from genome)Protein (477 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
HGNCMGIPubMedReactomeUniProtKBBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: TR61B_HUMAN
DESCRIPTION: RecName: Full=Potential tRNA (adenine(58)-N(1))-methyltransferase catalytic subunit TRMT61B; EC=2.1.1.220;
CATALYTIC ACTIVITY: S-adenosyl-L-methionine + adenine(58) in tRNA = S-adenosyl-L-homocysteine + N(1)-methyladenine(58) in tRNA.
INTERACTION: Q92985:IRF7; NbExp=2; IntAct=EBI-3197877, EBI-968267;
SIMILARITY: Belongs to the methyltransferase superfamily. TRM61 family.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
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-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 9.66 RPKM in Cells - EBV-transformed lymphocytes
Total median expression: 283.62 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
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-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -5.9032-0.184 Picture PostScript Text
3' UTR -57.50378-0.152 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR014816 - tRNA_MeTrfase_GCD14

Pfam Domains:
PF01135 - Protein-L-isoaspartate(D-aspartate) O-methyltransferase (PCMT)
PF08704 - tRNA methyltransferase complex GCD14 subunit

SCOP Domains:
51735 - NAD(P)-binding Rossmann-fold domains
53335 - S-adenosyl-L-methionine-dependent methyltransferases

Protein Data Bank (PDB) 3-D Structure
MuPIT help
2B25 - X-ray MuPIT


ModBase Predicted Comparative 3D Structure on Q9BVS5
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
      
      
  Ensembl   
      
      

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0005515 protein binding
GO:0008168 methyltransferase activity
GO:0016429 tRNA (adenine-N1-)-methyltransferase activity
GO:0016433 rRNA (adenine) methyltransferase activity
GO:0016740 transferase activity

Biological Process:
GO:0008033 tRNA processing
GO:0030488 tRNA methylation
GO:0031167 rRNA methylation
GO:0032259 methylation
GO:0051260 protein homooligomerization
GO:0070901 mitochondrial tRNA methylation
GO:0080009 mRNA methylation

Cellular Component:
GO:0005739 mitochondrion
GO:0005759 mitochondrial matrix
GO:0031515 tRNA (m1A) methyltransferase complex


-  Descriptions from all associated GenBank mRNAs
  AK308821 - Homo sapiens cDNA, FLJ98862.
AK225551 - Homo sapiens mRNA for hypothetical protein LOC55006 variant, clone: KDN03120.
BC000952 - Homo sapiens tRNA methyltransferase 61 homolog B (S. cerevisiae), mRNA (cDNA clone MGC:4955 IMAGE:3450078), complete cds.
BC010365 - Homo sapiens tRNA methyltransferase 61 homolog B (S. cerevisiae), mRNA (cDNA clone MGC:13425 IMAGE:4284118), complete cds.
AL136689 - Homo sapiens mRNA; cDNA DKFZp564I2178 (from clone DKFZp564I2178).
JD552855 - Sequence 533879 from Patent EP1572962.
AK000635 - Homo sapiens cDNA FLJ20628 fis, clone KAT03903.
AK225524 - Homo sapiens mRNA for hypothetical protein LOC55006 variant, clone: KAT03903.
JD224670 - Sequence 205694 from Patent EP1572962.
HQ448250 - Synthetic construct Homo sapiens clone IMAGE:100071651; CCSB006447_03 hypothetical protein FLJ20628 (FLJ20628) gene, encodes complete protein.
KJ899055 - Synthetic construct Homo sapiens clone ccsbBroadEn_08449 TRMT61B gene, encodes complete protein.
KJ899056 - Synthetic construct Homo sapiens clone ccsbBroadEn_08450 TRMT61B gene, encodes complete protein.
CU679681 - Synthetic construct Homo sapiens gateway clone IMAGE:100017346 5' read FLJ20628 mRNA.
CU674472 - Synthetic construct Homo sapiens gateway clone IMAGE:100019579 5' read FLJ20628 mRNA.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q9BVS5 (Reactome details) participates in the following event(s):

R-HSA-6787525 TRMT61B methylates adenosine-58 in tRNA yielding 1-methyladenosine-58
R-HSA-6787450 tRNA modification in the mitochondrion
R-HSA-72306 tRNA processing
R-HSA-8953854 Metabolism of RNA

-  Other Names for This Gene
  Alternate Gene Symbols: ENST00000306108.1, ENST00000306108.2, ENST00000306108.3, ENST00000306108.4, ENST00000306108.5, ENST00000306108.6, ENST00000306108.7, ENST00000306108.8, ENST00000306108.9, NM_017910, Q9BVS5, Q9H0Q9, Q9NWS7, TR61B_HUMAN, uc317ntt.1, uc317ntt.2
UCSC ID: ENST00000306108.10_7
RefSeq Accession: NM_017910.4
Protein: Q9BVS5 (aka TR61B_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.