Human Gene TRPC4AP (ENST00000252015.3_13) from GENCODE V47lift37
  Description: transient receptor potential cation channel subfamily C member 4 associated protein, transcript variant 1 (from RefSeq NM_015638.3)
Gencode Transcript: ENST00000252015.3_13
Gencode Gene: ENSG00000100991.12_15
Transcript (Including UTRs)
   Position: hg19 chr20:33,590,207-33,680,610 Size: 90,404 Total Exon Count: 19 Strand: -
Coding Region
   Position: hg19 chr20:33,590,949-33,680,584 Size: 89,636 Coding Exon Count: 19 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr20:33,590,207-33,680,610)mRNA (may differ from genome)Protein (797 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
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-  Comments and Description Text from UniProtKB
  ID: TP4AP_HUMAN
DESCRIPTION: RecName: Full=Short transient receptor potential channel 4-associated protein; Short=Trp4-associated protein; Short=Trpc4-associated protein; AltName: Full=Protein TAP1; AltName: Full=TNF-receptor ubiquitous scaffolding/signaling protein; Short=Protein TRUSS;
FUNCTION: Substrate-specific adapter of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control. The DCX(TRUSS) complex specifically mediates the polyubiquitination and subsequent degradation of MYC. Also participates in the activation of NFKB1 in response to ligation of TNFRSF1A, possibly by linking TNFRSF1A to the IKK signalosome. Involved in JNK activation via its interaction with TRAF2. Also involved in elevation of endoplasmic reticulum Ca(2+) storage reduction in response to CHRM1.
PATHWAY: Protein modification; protein ubiquitination.
SUBUNIT: Constitutively associated with TNFRSF1A. Directly interacts with TRADD, TRAF2, CHUK, IKBKB and IKBKG. Interacts with TRPC1, TRPC4 and TRPC5 (By similarity). Component of the DCX(TRUSS) E3 ubiquitin ligase complex, at least composed of CUL4A, DDB1, TRPC4AP/TRUSS and RBX1. Interacts with MYC.
INTERACTION: P62136:PPP1CA; NbExp=2; IntAct=EBI-2559060, EBI-357253;
SEQUENCE CAUTION: Sequence=BAB84932.1; Type=Frameshift; Positions=43;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 50.25 RPKM in Pituitary
Total median expression: 1692.18 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -5.1026-0.196 Picture PostScript Text
3' UTR -316.40742-0.426 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR022162 - DUF3689

Pfam Domains:
PF12463 - Protein of unknown function (DUF3689)

SCOP Domains:
48371 - ARM repeat

ModBase Predicted Comparative 3D Structure on Q8TEL6
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0005262 calcium channel activity
GO:0005515 protein binding
GO:0019902 phosphatase binding

Biological Process:
GO:0006511 ubiquitin-dependent protein catabolic process
GO:0016567 protein ubiquitination
GO:0048820 hair follicle maturation
GO:0070588 calcium ion transmembrane transport

Cellular Component:
GO:0005886 plasma membrane
GO:0031464 Cul4A-RING E3 ubiquitin ligase complex


-  Descriptions from all associated GenBank mRNAs
  LF350273 - JP 2014500723-A/157776: Polycomb-Associated Non-Coding RNAs.
BC008836 - Homo sapiens transient receptor potential cation channel, subfamily C, member 4 associated protein, mRNA (cDNA clone IMAGE:3530893), partial cds.
LF383754 - JP 2014500723-A/191257: Polycomb-Associated Non-Coding RNAs.
AL096738 - Homo sapiens mRNA; cDNA DKFZp586C1223 (from clone DKFZp586C1223).
BC001323 - Homo sapiens transient receptor potential cation channel, subfamily C, member 4 associated protein, mRNA (cDNA clone IMAGE:3456260), partial cds.
AF055022 - Homo sapiens clone 24684 mRNA sequence.
AL117480 - Homo sapiens mRNA; cDNA DKFZp727M231 (from clone DKFZp727M231).
BC013144 - Homo sapiens transient receptor potential cation channel, subfamily C, member 4 associated protein, mRNA (cDNA clone MGC:21388 IMAGE:4475866), complete cds.
AK074106 - Homo sapiens mRNA for FLJ00177 protein.
JD537044 - Sequence 518068 from Patent EP1572962.
JD474934 - Sequence 455958 from Patent EP1572962.
JD477793 - Sequence 458817 from Patent EP1572962.
JD219052 - Sequence 200076 from Patent EP1572962.
JD157363 - Sequence 138387 from Patent EP1572962.
JD554749 - Sequence 535773 from Patent EP1572962.
JD532383 - Sequence 513407 from Patent EP1572962.
JD226739 - Sequence 207763 from Patent EP1572962.
AK301368 - Homo sapiens cDNA FLJ50256 complete cds, highly similar to Trpc4-associated protein.
JD142743 - Sequence 123767 from Patent EP1572962.
JD283857 - Sequence 264881 from Patent EP1572962.
JD563464 - Sequence 544488 from Patent EP1572962.
JD384167 - Sequence 365191 from Patent EP1572962.
JD216536 - Sequence 197560 from Patent EP1572962.
JD393503 - Sequence 374527 from Patent EP1572962.
AK303476 - Homo sapiens cDNA FLJ50335 complete cds, highly similar to Trpc4-associated protein.
JD521017 - Sequence 502041 from Patent EP1572962.
AK298958 - Homo sapiens cDNA FLJ51744 complete cds, highly similar to Trpc4-associated protein.
JD200684 - Sequence 181708 from Patent EP1572962.
JD271154 - Sequence 252178 from Patent EP1572962.
JD392793 - Sequence 373817 from Patent EP1572962.
AK315582 - Homo sapiens cDNA, FLJ96658, highly similar to Homo sapiens transient receptor potential cation channel, subfamilyC, member 4 associated protein (TRPC4AP), mRNA.
JF432334 - Synthetic construct Homo sapiens clone IMAGE:100073521 transient receptor potential cation channel, subfamily C, member 4 associated protein (TRPC4AP) gene, encodes complete protein.
KJ898597 - Synthetic construct Homo sapiens clone ccsbBroadEn_07991 TRPC4AP gene, encodes complete protein.
AB590540 - Synthetic construct DNA, clone: pFN21AB5739, Homo sapiens TRPC4AP gene for transient receptor potential cation channel, subfamily C, member 4 associated protein, without stop codon, in Flexi system.
LF350275 - JP 2014500723-A/157778: Polycomb-Associated Non-Coding RNAs.
LF350276 - JP 2014500723-A/157779: Polycomb-Associated Non-Coding RNAs.
LF350279 - JP 2014500723-A/157782: Polycomb-Associated Non-Coding RNAs.
LF350281 - JP 2014500723-A/157784: Polycomb-Associated Non-Coding RNAs.
LF350282 - JP 2014500723-A/157785: Polycomb-Associated Non-Coding RNAs.
CU680522 - Synthetic construct Homo sapiens gateway clone IMAGE:100020542 5' read TRPC4AP mRNA.
LF350285 - JP 2014500723-A/157788: Polycomb-Associated Non-Coding RNAs.
LF350288 - JP 2014500723-A/157791: Polycomb-Associated Non-Coding RNAs.
MA585850 - JP 2018138019-A/157776: Polycomb-Associated Non-Coding RNAs.
MA619331 - JP 2018138019-A/191257: Polycomb-Associated Non-Coding RNAs.
MA585852 - JP 2018138019-A/157778: Polycomb-Associated Non-Coding RNAs.
MA585853 - JP 2018138019-A/157779: Polycomb-Associated Non-Coding RNAs.
MA585856 - JP 2018138019-A/157782: Polycomb-Associated Non-Coding RNAs.
MA585858 - JP 2018138019-A/157784: Polycomb-Associated Non-Coding RNAs.
MA585859 - JP 2018138019-A/157785: Polycomb-Associated Non-Coding RNAs.
MA585862 - JP 2018138019-A/157788: Polycomb-Associated Non-Coding RNAs.
MA585865 - JP 2018138019-A/157791: Polycomb-Associated Non-Coding RNAs.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q8TEL6 (Reactome details) participates in the following event(s):

R-HSA-3295579 TRPs transport extracellular Ca2+ to cytosol
R-HSA-3295583 TRP channels
R-HSA-2672351 Stimuli-sensing channels
R-HSA-983712 Ion channel transport
R-HSA-382551 Transport of small molecules

-  Other Names for This Gene
  Alternate Gene Symbols: C20orf188 , E1P5Q0, E1P5Q1, ENST00000252015.1, ENST00000252015.2, NM_015638, Q8TEL6, Q96H82, Q9BVB8, Q9H429, Q9UFS6, TP4AP_HUMAN, TRPC4AP , TRRP4AP, uc317ffr.1, uc317ffr.2
UCSC ID: ENST00000252015.3_13
RefSeq Accession: NM_015638.3
Protein: Q8TEL6 (aka TP4AP_HUMAN or T4AP_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.