ID:U2AF2_HUMAN DESCRIPTION: RecName: Full=Splicing factor U2AF 65 kDa subunit; AltName: Full=U2 auxiliary factor 65 kDa subunit; Short=hU2AF(65); Short=hU2AF65; AltName: Full=U2 snRNP auxiliary factor large subunit; FUNCTION: Necessary for the splicing of pre-mRNA. Induces cardiac troponin-T (TNNT2) pre-mRNA exon inclusion in muscle. Regulates the TNNT2 exon 5 inclusion through competition with MBNL1. Binds preferentially to a single-stranded structure within the polypyrimidine tract of TNNT2 intron 4 during spliceosome assembly. Required for the export of mRNA out of the nucleus, even if the mRNA is encoded by an intron-less gene. Represses the splicing of MAPT/Tau exon 10. SUBUNIT: Interacts with U2AF1L4 (By similarity). Heterodimer with U2AF1. Binds unphosphorylated SF1. Interacts with SCAF11. Interacts with ZRSR2/U2AF1-RS2. INTERACTION: P54253:ATXN1; NbExp=4; IntAct=EBI-742339, EBI-930964; Q01081:U2AF1; NbExp=3; IntAct=EBI-742339, EBI-632461; SUBCELLULAR LOCATION: Nucleus. PTM: Lysyl-hydroxylation at Lys-15 and Lys-276 affects the mRNA splicing activity of the protein, leading to regulate some, but not all, alternative splicing events. SIMILARITY: Belongs to the splicing factor SR family. SIMILARITY: Contains 3 RRM (RNA recognition motif) domains.
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on P26368
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
BC030574 - Homo sapiens U2 small nuclear RNA auxiliary factor 2, mRNA (cDNA clone MGC:16243 IMAGE:3687471), complete cds. BC008740 - Homo sapiens U2 small nuclear RNA auxiliary factor 2, mRNA (cDNA clone MGC:1210 IMAGE:3346087), complete cds. X64044 - H.sapiens mmRNA for large subunit of splicing factor U2AF. CU679069 - Synthetic construct Homo sapiens gateway clone IMAGE:100019553 5' read U2AF2 mRNA. JF432180 - Synthetic construct Homo sapiens clone IMAGE:100073335 U2 small nuclear RNA auxiliary factor 2 (U2AF2) gene, encodes complete protein. KJ898405 - Synthetic construct Homo sapiens clone ccsbBroadEn_07799 U2AF2 gene, encodes complete protein. AB527505 - Synthetic construct DNA, clone: pF1KB5815, Homo sapiens U2AF2 gene for U2 small nuclear RNA auxiliary factor 2, without stop codon, in Flexi system. AB451387 - Homo sapiens U2AF2 mRNA for U2 small nuclear RNA auxiliary factor 2 isoform b, partial cds, clone: FLJ08076AAAF. AB451261 - Homo sapiens U2AF2 mRNA for U2 small nuclear RNA auxiliary factor 2 isoform b, complete cds, clone: FLJ08076AAAN. DQ592840 - Homo sapiens piRNA piR-59952, complete sequence. JD453386 - Sequence 434410 from Patent EP1572962. JD436153 - Sequence 417177 from Patent EP1572962. JD329669 - Sequence 310693 from Patent EP1572962. JD206235 - Sequence 187259 from Patent EP1572962. JD499333 - Sequence 480357 from Patent EP1572962. JD552176 - Sequence 533200 from Patent EP1572962.
Biochemical and Signaling Pathways
Reactome (by CSHL, EBI, and GO)
Protein P26368 (Reactome details) participates in the following event(s):
R-HSA-72107 Formation of the Spliceosomal E complex R-HSA-75096 Docking of the TAP:EJC Complex with the NPC R-HSA-72143 Lariat Formation and 5'-Splice Site Cleavage R-HSA-72139 Formation of the active Spliceosomal C (B*) complex R-HSA-72124 Formation of the Spliceosomal A Complex R-HSA-72127 Formation of the Spliceosomal B Complex R-HSA-72130 Formation of an intermediate Spliceosomal C (Bact) complex R-HSA-8849157 TREX complex binds spliced, capped mRNA:CBC:EJC cotranscriptionally R-HSA-72185 mRNA polyadenylation R-HSA-72180 Cleavage of mRNA at the 3'-end R-HSA-156661 Formation of Exon Junction Complex R-HSA-159101 NXF1:NXT1 (TAP:p15) binds capped mRNA:CBC:EJC:TREX (minus DDX39B) R-HSA-72163 mRNA Splicing - Major Pathway R-HSA-159236 Transport of Mature mRNA derived from an Intron-Containing Transcript R-HSA-72187 mRNA 3'-end processing R-HSA-109688 Cleavage of Growing Transcript in the Termination Region R-HSA-72172 mRNA Splicing R-HSA-72202 Transport of Mature Transcript to Cytoplasm R-HSA-72203 Processing of Capped Intron-Containing Pre-mRNA R-HSA-73856 RNA Polymerase II Transcription Termination R-HSA-8953854 Metabolism of RNA R-HSA-73857 RNA Polymerase II Transcription R-HSA-74160 Gene expression (Transcription)
US Server
Sequence and Links to Tools and Databases 
Common Gene Haplotype Alleles 
