Human Gene UGDH (ENST00000316423.11_7) from GENCODE V47lift37
  Description: UDP-glucose 6-dehydrogenase, transcript variant 1 (from RefSeq NM_003359.4)
Gencode Transcript: ENST00000316423.11_7
Gencode Gene: ENSG00000109814.12_11
Transcript (Including UTRs)
   Position: hg19 chr4:39,500,375-39,529,059 Size: 28,685 Total Exon Count: 12 Strand: -
Coding Region
   Position: hg19 chr4:39,501,763-39,523,132 Size: 21,370 Coding Exon Count: 11 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr4:39,500,375-39,529,059)mRNA (may differ from genome)Protein (494 aa)
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BioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: UGDH_HUMAN
DESCRIPTION: RecName: Full=UDP-glucose 6-dehydrogenase; Short=UDP-Glc dehydrogenase; Short=UDP-GlcDH; Short=UDPGDH; EC=1.1.1.22;
FUNCTION: Involved in the biosynthesis of glycosaminoglycans; hyaluronan, chondroitin sulfate, and heparan sulfate.
CATALYTIC ACTIVITY: UDP-glucose + 2 NAD(+) + H(2)O = UDP- glucuronate + 2 NADH.
ENZYME REGULATION: UDP-alpha-D-xylose (UDX) acts as a feedback inhibitor by activating an allosteric switch.
PATHWAY: Nucleotide-sugar biosynthesis; UDP-alpha-D-glucuronate biosynthesis; UDP-alpha-D-glucuronate from UDP-alpha-D-glucose: step 1/1.
SUBUNIT: Homohexamer.
TISSUE SPECIFICITY: Widely expressed.
SIMILARITY: Belongs to the UDP-glucose/GDP-mannose dehydrogenase family.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
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-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 30.73 RPKM in Colon - Transverse
Total median expression: 404.09 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
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-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -66.40164-0.405 Picture PostScript Text
3' UTR -313.501388-0.226 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR008927 - 6-PGluconate_DH_C-like
IPR021157 - Cyt_c1_TM_anchor_C
IPR016040 - NAD(P)-bd_dom
IPR017476 - Nucleotide_sugar_DH
IPR014027 - UDP-Glc/GDP-Man_DH_C
IPR014026 - UDP-Glc/GDP-Man_DH_dimer
IPR001732 - UDP-Glc/GDP-Man_DH_N

Pfam Domains:
PF00984 - UDP-glucose/GDP-mannose dehydrogenase family, central domain
PF03720 - UDP-glucose/GDP-mannose dehydrogenase family, UDP binding domain
PF03721 - UDP-glucose/GDP-mannose dehydrogenase family, NAD binding domain

SCOP Domains:
48179 - 6-phosphogluconate dehydrogenase C-terminal domain-like
51735 - NAD(P)-binding Rossmann-fold domains
52413 - UDP-glucose/GDP-mannose dehydrogenase C-terminal domain
51905 - FAD/NAD(P)-binding domain
51971 - Nucleotide-binding domain
51984 - MurCD N-terminal domain

Protein Data Bank (PDB) 3-D Structure
MuPIT help
2Q3E - X-ray MuPIT 2QG4 - X-ray MuPIT 3ITK - X-ray MuPIT 3KHU - X-ray MuPIT 3PRJ - X-ray MuPIT 3PTZ - X-ray MuPIT 3TDK - X-ray MuPIT 3TF5 - X-ray MuPIT


ModBase Predicted Comparative 3D Structure on O60701
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0003824 catalytic activity
GO:0003979 UDP-glucose 6-dehydrogenase activity
GO:0009055 electron carrier activity
GO:0016491 oxidoreductase activity
GO:0016616 oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
GO:0051287 NAD binding

Biological Process:
GO:0001702 gastrulation with mouth forming second
GO:0006011 UDP-glucose metabolic process
GO:0006024 glycosaminoglycan biosynthetic process
GO:0006065 UDP-glucuronate biosynthetic process
GO:0008152 metabolic process
GO:0022900 electron transport chain
GO:0055114 oxidation-reduction process

Cellular Component:
GO:0005634 nucleus
GO:0005654 nucleoplasm
GO:0005829 cytosol
GO:0070062 extracellular exosome


-  Descriptions from all associated GenBank mRNAs
  LP747441 - Sequence 28 from Patent WO2018009939.
BC013430 - Homo sapiens, clone IMAGE:4051555, mRNA.
AF061016 - Homo sapiens UDP-glucose dehydrogenase (UGDH) mRNA, complete cds.
AK097930 - Homo sapiens cDNA FLJ40611 fis, clone THYMU2012690, highly similar to UDP-GLUCOSE 6-DEHYDROGENASE (EC 1.1.1.22).
JD449146 - Sequence 430170 from Patent EP1572962.
JD502367 - Sequence 483391 from Patent EP1572962.
JD242971 - Sequence 223995 from Patent EP1572962.
JD562958 - Sequence 543982 from Patent EP1572962.
AJ007702 - Homo sapiens mRNA for UDP-glucose 6-dehydrogenase.
BC022781 - Homo sapiens UDP-glucose dehydrogenase, mRNA (cDNA clone MGC:9321 IMAGE:3916854), complete cds.
JD288625 - Sequence 269649 from Patent EP1572962.
JD528716 - Sequence 509740 from Patent EP1572962.
JD264892 - Sequence 245916 from Patent EP1572962.
JD254781 - Sequence 235805 from Patent EP1572962.
JD513375 - Sequence 494399 from Patent EP1572962.
JD242631 - Sequence 223655 from Patent EP1572962.
AK297737 - Homo sapiens cDNA FLJ60093 complete cds, highly similar to UDP-glucose 6-dehydrogenase (EC 1.1.1.22).
JD213280 - Sequence 194304 from Patent EP1572962.
JD407651 - Sequence 388675 from Patent EP1572962.
JD256831 - Sequence 237855 from Patent EP1572962.
JD059649 - Sequence 40673 from Patent EP1572962.
JD152327 - Sequence 133351 from Patent EP1572962.
JD258806 - Sequence 239830 from Patent EP1572962.
DQ891116 - Synthetic construct clone IMAGE:100003746; FLH169454.01X; RZPDo839C0796D UDP-glucose dehydrogenase (UGDH) gene, encodes complete protein.
DQ894295 - Synthetic construct Homo sapiens clone IMAGE:100008755; FLH169450.01L; RZPDo839C0795D UDP-glucose dehydrogenase (UGDH) gene, encodes complete protein.
AB527640 - Synthetic construct DNA, clone: pF1KB5490, Homo sapiens UGDH gene for UDP-glucose dehydrogenase, without stop codon, in Flexi system.
AF049126 - Homo sapiens UDP glucose 6-dehydrogenase (UDPGDH) mRNA, partial cds.
CU692708 - Synthetic construct Homo sapiens gateway clone IMAGE:100019662 5' read UGDH mRNA.
MB486581 - JP 2019531699-A/28: METHODS FOR DIAGNOSING AND TREATING CANCER.

-  Biochemical and Signaling Pathways
  BioCyc Knowledge Library
PWY-4821 - UDP-α-D-xylose biosynthesis
PWY-7346 - UDP-α-D-glucuronate biosynthesis (from UDP-glucose)

Reactome (by CSHL, EBI, and GO)

Protein O60701 (Reactome details) participates in the following event(s):

R-HSA-173597 UDP-glucose is oxidised to UDP-glucuronate
R-HSA-173599 Formation of the active cofactor, UDP-glucuronate
R-HSA-156588 Glucuronidation
R-HSA-156580 Phase II - Conjugation of compounds
R-HSA-211859 Biological oxidations
R-HSA-1430728 Metabolism

-  Other Names for This Gene
  Alternate Gene Symbols: B3KUU2, B4DN25, ENST00000316423.1, ENST00000316423.10, ENST00000316423.2, ENST00000316423.3, ENST00000316423.4, ENST00000316423.5, ENST00000316423.6, ENST00000316423.7, ENST00000316423.8, ENST00000316423.9, NM_003359, O60589, O60701, uc317qad.1, uc317qad.2, UGDH_HUMAN
UCSC ID: ENST00000316423.11_7
RefSeq Accession: NM_003359.4
Protein: O60701 (aka UGDH_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.