Human Gene USP34 (ENST00000398571.7_8) from GENCODE V47lift37
  Description: ubiquitin specific peptidase 34 (from RefSeq NM_014709.4)
Gencode Transcript: ENST00000398571.7_8
Gencode Gene: ENSG00000115464.15_18
Transcript (Including UTRs)
   Position: hg19 chr2:61,414,598-61,698,222 Size: 283,625 Total Exon Count: 80 Strand: -
Coding Region
   Position: hg19 chr2:61,415,237-61,697,827 Size: 282,591 Coding Exon Count: 80 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr2:61,414,598-61,698,222)mRNA (may differ from genome)Protein (3546 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
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MGIOMIMPubMedReactomeUniProtKBWikipedia
BioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: UBP34_HUMAN
DESCRIPTION: RecName: Full=Ubiquitin carboxyl-terminal hydrolase 34; EC=3.4.19.12; AltName: Full=Deubiquitinating enzyme 34; AltName: Full=Ubiquitin thioesterase 34; AltName: Full=Ubiquitin-specific-processing protease 34;
FUNCTION: Ubiquitin hydrolase that can remove conjugated ubiquitin from AXIN1 and AXIN2, thereby acting as a regulator of Wnt signaling pathway. Acts as an activator of the Wnt signaling pathway downstream of the beta-catenin destruction complex by deubiquitinating and stabilizing AXIN1 and AXIN2, leading to promote nuclear accumulation of AXIN1 and AXIN2 and positively regulate beta-catenin (CTNBB1)-mediated transcription. Recognizes and hydrolyzes the peptide bond at the C-terminal Gly of ubiquitin. Involved in the processing of poly-ubiquitin precursors as well as that of ubiquitinated proteins.
CATALYTIC ACTIVITY: Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C- terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
SUBUNIT: Interacts with AXIN1 and AXIN2.
TISSUE SPECIFICITY: Expressed in brain at low level.
PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.
SIMILARITY: Belongs to the peptidase C19 family.
SEQUENCE CAUTION: Sequence=AAI07762.1; Type=Frameshift; Positions=3200; Sequence=BAA25496.2; Type=Erroneous initiation; Sequence=BAG54261.1; Type=Erroneous initiation;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 16.67 RPKM in Cells - EBV-transformed lymphocytes
Total median expression: 396.72 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -159.75395-0.404 Picture PostScript Text
3' UTR -148.40639-0.232 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR016024 - ARM-type_fold
IPR018200 - Pept_C19ubi-hydrolase_C_CS
IPR001394 - Peptidase_C19

Pfam Domains:
PF00443 - Ubiquitin carboxyl-terminal hydrolase
PF12030 - Domain of unknown function (DUF3517)
PF13423 - Ubiquitin carboxyl-terminal hydrolase

SCOP Domains:
48371 - ARM repeat
52833 - Thioredoxin-like
54001 - Cysteine proteinases

ModBase Predicted Comparative 3D Structure on Q70CQ2
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details Gene Details  
Gene SorterGene Sorter Gene Sorter  
 RGDEnsembl   
      
      

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0004197 cysteine-type endopeptidase activity
GO:0004843 thiol-dependent ubiquitin-specific protease activity
GO:0005515 protein binding
GO:0008233 peptidase activity
GO:0008234 cysteine-type peptidase activity
GO:0016787 hydrolase activity
GO:0036459 thiol-dependent ubiquitinyl hydrolase activity

Biological Process:
GO:0006508 proteolysis
GO:0006511 ubiquitin-dependent protein catabolic process
GO:0016055 Wnt signaling pathway
GO:0016579 protein deubiquitination
GO:0071108 protein K48-linked deubiquitination
GO:0090263 positive regulation of canonical Wnt signaling pathway

Cellular Component:
GO:0005829 cytosol


-  Descriptions from all associated GenBank mRNAs
  BC141839 - Homo sapiens ubiquitin specific peptidase 34, mRNA (cDNA clone IMAGE:8860123).
AB011142 - Homo sapiens mRNA for KIAA0570 protein, partial cds.
AJ586138 - Homo sapiens mRNA for ubiquitin-specific proteinase 34 (USP34 gene).
AK125898 - Homo sapiens cDNA FLJ43910 fis, clone TESTI4010851, highly similar to Homo sapiens ubiquitin specific peptidase 34 (USP34), mRNA.
AK023845 - Homo sapiens cDNA FLJ13783 fis, clone PLACE4000558, weakly similar to PROBABLE UBIQUITIN CARBOXYL-TERMINAL HYDROLASE FAF (EC 3.1.2.15).
AL831918 - Homo sapiens mRNA; cDNA DKFZp761F2418 (from clone DKFZp761F2418).
BC022783 - Homo sapiens ubiquitin specific peptidase 34, mRNA (cDNA clone IMAGE:3846921), partial cds.
AK023171 - Homo sapiens cDNA FLJ13109 fis, clone NT2RP3002545, highly similar to Homo sapiens mRNA; cDNA DKFZp586G0518 (from clone DKFZp586G0518).
BC107761 - Homo sapiens ubiquitin specific peptidase 34, mRNA (cDNA clone IMAGE:4612408), complete cds.
AK001193 - Homo sapiens cDNA FLJ10331 fis, clone NT2RM2000635, highly similar to Homo sapiens mRNA for KIAA0729 protein.
AK022637 - Homo sapiens cDNA FLJ12575 fis, clone NT2RM4001002, highly similar to Homo sapiens mRNA; cDNA DKFZp586G0518 (from clone DKFZp586G0518).
BC062325 - Homo sapiens ubiquitin specific peptidase 34, mRNA (cDNA clone IMAGE:5426830), complete cds.
AL050092 - Homo sapiens mRNA; cDNA DKFZp586G0518 (from clone DKFZp586G0518).
AB018272 - Homo sapiens mRNA for KIAA0729 protein, partial cds.
LF325139 - JP 2014500723-A/132642: Polycomb-Associated Non-Coding RNAs.
JD350224 - Sequence 331248 from Patent EP1572962.
JD226255 - Sequence 207279 from Patent EP1572962.
JD373839 - Sequence 354863 from Patent EP1572962.
LF325138 - JP 2014500723-A/132641: Polycomb-Associated Non-Coding RNAs.
LF325137 - JP 2014500723-A/132640: Polycomb-Associated Non-Coding RNAs.
JD079748 - Sequence 60772 from Patent EP1572962.
JD232223 - Sequence 213247 from Patent EP1572962.
JD109690 - Sequence 90714 from Patent EP1572962.
JD166551 - Sequence 147575 from Patent EP1572962.
JD565074 - Sequence 546098 from Patent EP1572962.
JD199201 - Sequence 180225 from Patent EP1572962.
JD524156 - Sequence 505180 from Patent EP1572962.
JD037951 - Sequence 18975 from Patent EP1572962.
JD515500 - Sequence 496524 from Patent EP1572962.
JD491561 - Sequence 472585 from Patent EP1572962.
LF325136 - JP 2014500723-A/132639: Polycomb-Associated Non-Coding RNAs.
JD120762 - Sequence 101786 from Patent EP1572962.
JD532888 - Sequence 513912 from Patent EP1572962.
LF325133 - JP 2014500723-A/132636: Polycomb-Associated Non-Coding RNAs.
JD103564 - Sequence 84588 from Patent EP1572962.
JD155523 - Sequence 136547 from Patent EP1572962.
JD366853 - Sequence 347877 from Patent EP1572962.
JD421777 - Sequence 402801 from Patent EP1572962.
AK126741 - Homo sapiens cDNA FLJ44787 fis, clone BRACE3038569.
AK124932 - Homo sapiens cDNA FLJ42942 fis, clone BRSTN2001067.
JD326458 - Sequence 307482 from Patent EP1572962.
JD088657 - Sequence 69681 from Patent EP1572962.
JD298764 - Sequence 279788 from Patent EP1572962.
JD359457 - Sequence 340481 from Patent EP1572962.
JD131050 - Sequence 112074 from Patent EP1572962.
JD091343 - Sequence 72367 from Patent EP1572962.
JD162868 - Sequence 143892 from Patent EP1572962.
JD080085 - Sequence 61109 from Patent EP1572962.
LF325125 - JP 2014500723-A/132628: Polycomb-Associated Non-Coding RNAs.
JD095733 - Sequence 76757 from Patent EP1572962.
JD467219 - Sequence 448243 from Patent EP1572962.
JD501307 - Sequence 482331 from Patent EP1572962.
JD090897 - Sequence 71921 from Patent EP1572962.
JD494714 - Sequence 475738 from Patent EP1572962.
JD496513 - Sequence 477537 from Patent EP1572962.
JD427734 - Sequence 408758 from Patent EP1572962.
JD295294 - Sequence 276318 from Patent EP1572962.
JD468399 - Sequence 449423 from Patent EP1572962.
JD468386 - Sequence 449410 from Patent EP1572962.
JD248272 - Sequence 229296 from Patent EP1572962.
JD223449 - Sequence 204473 from Patent EP1572962.
JD455168 - Sequence 436192 from Patent EP1572962.
LF325124 - JP 2014500723-A/132627: Polycomb-Associated Non-Coding RNAs.
LF325123 - JP 2014500723-A/132626: Polycomb-Associated Non-Coding RNAs.
JD368541 - Sequence 349565 from Patent EP1572962.
JD356753 - Sequence 337777 from Patent EP1572962.
LF325122 - JP 2014500723-A/132625: Polycomb-Associated Non-Coding RNAs.
JD454952 - Sequence 435976 from Patent EP1572962.
JD316090 - Sequence 297114 from Patent EP1572962.
LF325121 - JP 2014500723-A/132624: Polycomb-Associated Non-Coding RNAs.
JD431539 - Sequence 412563 from Patent EP1572962.
MA560716 - JP 2018138019-A/132642: Polycomb-Associated Non-Coding RNAs.
MA560715 - JP 2018138019-A/132641: Polycomb-Associated Non-Coding RNAs.
MA560714 - JP 2018138019-A/132640: Polycomb-Associated Non-Coding RNAs.
MA560713 - JP 2018138019-A/132639: Polycomb-Associated Non-Coding RNAs.
MA560710 - JP 2018138019-A/132636: Polycomb-Associated Non-Coding RNAs.
MA560702 - JP 2018138019-A/132628: Polycomb-Associated Non-Coding RNAs.
MA560701 - JP 2018138019-A/132627: Polycomb-Associated Non-Coding RNAs.
MA560700 - JP 2018138019-A/132626: Polycomb-Associated Non-Coding RNAs.
MA560699 - JP 2018138019-A/132625: Polycomb-Associated Non-Coding RNAs.
MA560698 - JP 2018138019-A/132624: Polycomb-Associated Non-Coding RNAs.
LF325120 - JP 2014500723-A/132623: Polycomb-Associated Non-Coding RNAs.
JD062831 - Sequence 43855 from Patent EP1572962.
JD528536 - Sequence 509560 from Patent EP1572962.
LF325119 - JP 2014500723-A/132622: Polycomb-Associated Non-Coding RNAs.
LF325118 - JP 2014500723-A/132621: Polycomb-Associated Non-Coding RNAs.
LF325117 - JP 2014500723-A/132620: Polycomb-Associated Non-Coding RNAs.
LF325116 - JP 2014500723-A/132619: Polycomb-Associated Non-Coding RNAs.
JD241297 - Sequence 222321 from Patent EP1572962.
JD164964 - Sequence 145988 from Patent EP1572962.
LF325112 - JP 2014500723-A/132615: Polycomb-Associated Non-Coding RNAs.
LF325110 - JP 2014500723-A/132613: Polycomb-Associated Non-Coding RNAs.
JD110950 - Sequence 91974 from Patent EP1572962.
JD364230 - Sequence 345254 from Patent EP1572962.
JD364227 - Sequence 345251 from Patent EP1572962.
LF325109 - JP 2014500723-A/132612: Polycomb-Associated Non-Coding RNAs.
LF325108 - JP 2014500723-A/132611: Polycomb-Associated Non-Coding RNAs.
LF325107 - JP 2014500723-A/132610: Polycomb-Associated Non-Coding RNAs.
MA560697 - JP 2018138019-A/132623: Polycomb-Associated Non-Coding RNAs.
MA560696 - JP 2018138019-A/132622: Polycomb-Associated Non-Coding RNAs.
MA560695 - JP 2018138019-A/132621: Polycomb-Associated Non-Coding RNAs.
MA560694 - JP 2018138019-A/132620: Polycomb-Associated Non-Coding RNAs.
MA560693 - JP 2018138019-A/132619: Polycomb-Associated Non-Coding RNAs.
MA560689 - JP 2018138019-A/132615: Polycomb-Associated Non-Coding RNAs.
MA560687 - JP 2018138019-A/132613: Polycomb-Associated Non-Coding RNAs.
MA560686 - JP 2018138019-A/132612: Polycomb-Associated Non-Coding RNAs.
MA560685 - JP 2018138019-A/132611: Polycomb-Associated Non-Coding RNAs.
MA560684 - JP 2018138019-A/132610: Polycomb-Associated Non-Coding RNAs.
LF325103 - JP 2014500723-A/132606: Polycomb-Associated Non-Coding RNAs.
LF325102 - JP 2014500723-A/132605: Polycomb-Associated Non-Coding RNAs.
LF325100 - JP 2014500723-A/132603: Polycomb-Associated Non-Coding RNAs.
MA560680 - JP 2018138019-A/132606: Polycomb-Associated Non-Coding RNAs.
MA560679 - JP 2018138019-A/132605: Polycomb-Associated Non-Coding RNAs.
MA560677 - JP 2018138019-A/132603: Polycomb-Associated Non-Coding RNAs.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q70CQ2 (Reactome details) participates in the following event(s):

R-HSA-3640872 USP34 deubiquitinates AXIN1,AXIN2
R-HSA-201681 TCF dependent signaling in response to WNT
R-HSA-5689880 Ub-specific processing proteases
R-HSA-195721 Signaling by WNT
R-HSA-5688426 Deubiquitination
R-HSA-162582 Signal Transduction
R-HSA-597592 Post-translational protein modification
R-HSA-392499 Metabolism of proteins

-  Other Names for This Gene
  Alternate Gene Symbols: A8MWD0, B3KWU9, ENST00000398571.1, ENST00000398571.2, ENST00000398571.3, ENST00000398571.4, ENST00000398571.5, ENST00000398571.6, KIAA0570, KIAA0729, NM_014709, O60316, O94834, Q3B777, Q6P6C9, Q70CQ2, Q7L8P6, Q8N3T9, Q8TBW2, Q9UGA1, UBP34_HUMAN, uc318zuu.1, uc318zuu.2
UCSC ID: ENST00000398571.7_8
RefSeq Accession: NM_014709.4
Protein: Q70CQ2 (aka UBP34_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.