Human Gene WDR74 (ENST00000278856.9_8) from GENCODE V47lift37
  Description: WD repeat domain 74, transcript variant 11 (from RefSeq NR_161381.1)
Gencode Transcript: ENST00000278856.9_8
Gencode Gene: ENSG00000133316.16_17
Transcript (Including UTRs)
   Position: hg19 chr11:62,600,383-62,607,062 Size: 6,680 Total Exon Count: 11 Strand: -
Coding Region
   Position: hg19 chr11:62,600,424-62,607,042 Size: 6,619 Coding Exon Count: 11 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr11:62,600,383-62,607,062)mRNA (may differ from genome)Protein (385 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
HGNCMGIOMIMPubMedUniProtKBBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: WDR74_HUMAN
DESCRIPTION: RecName: Full=WD repeat-containing protein 74; AltName: Full=NOP seven-associated protein 1;
SUBCELLULAR LOCATION: Nucleus, nucleolus.
SIMILARITY: Contains 6 WD repeats.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 15.98 RPKM in Testis
Total median expression: 472.40 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -4.5020-0.225 Picture PostScript Text
3' UTR -0.8041-0.020 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR015943 - WD40/YVTN_repeat-like_dom
IPR001680 - WD40_repeat
IPR017986 - WD40_repeat_dom

Pfam Domains:
PF00400 - WD domain, G-beta repeat

SCOP Domains:
50952 - Soluble quinoprotein glucose dehydrogenase
50956 - Thermostable phytase (3-phytase)
50960 - TolB, C-terminal domain
63825 - YWTD domain
63829 - Calcium-dependent phosphotriesterase
69304 - Tricorn protease N-terminal domain
101898 - NHL repeat
50965 - Galactose oxidase, central domain
75011 - 3-carboxy-cis,cis-mucoante lactonizing enzyme
101908 - Putative isomerase YbhE
117289 - Nucleoporin domain
50969 - YVTN repeat-like/Quinoprotein amine dehydrogenase
50974 - Nitrous oxide reductase, N-terminal domain
50978 - WD40 repeat-like
50993 - Peptidase/esterase 'gauge' domain
69322 - Tricorn protease domain 2
50998 - Quinoprotein alcohol dehydrogenase-like
51004 - C-terminal (heme d1) domain of cytochrome cd1-nitrite reductase
82171 - DPP6 N-terminal domain-like

ModBase Predicted Comparative 3D Structure on Q6RFH5
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
Gene DetailsGene Details    
Gene SorterGene Sorter    
 RGD    
      
      

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0003674 molecular_function
GO:0005515 protein binding

Biological Process:
GO:0008150 biological_process
GO:0042273 ribosomal large subunit biogenesis

Cellular Component:
GO:0005634 nucleus
GO:0005730 nucleolus
GO:0030687 preribosome, large subunit precursor


-  Descriptions from all associated GenBank mRNAs
  LF211226 - JP 2014500723-A/18729: Polycomb-Associated Non-Coding RNAs.
AK025383 - Homo sapiens cDNA: FLJ21730 fis, clone COLF1557.
AY500828 - Homo sapiens NOP seven associated protein 1 (NSA1) mRNA, complete cds.
BC006351 - Homo sapiens WD repeat domain 74, mRNA (cDNA clone MGC:12596 IMAGE:4111690), complete cds.
AK001301 - Homo sapiens cDNA FLJ10439 fis, clone NT2RP1000688.
AK292330 - Homo sapiens cDNA FLJ78749 complete cds, highly similar to Homo sapiens WD repeat domain 74 (WDR74), mRNA.
HQ447879 - Synthetic construct Homo sapiens clone IMAGE:100071229; CCSB004913_02 WD repeat domain 74 (WDR74) gene, encodes complete protein.
KJ902676 - Synthetic construct Homo sapiens clone ccsbBroadEn_12070 WDR74 gene, encodes complete protein.
LF344739 - JP 2014500723-A/152242: Polycomb-Associated Non-Coding RNAs.
LF344738 - JP 2014500723-A/152241: Polycomb-Associated Non-Coding RNAs.
LF344736 - JP 2014500723-A/152239: Polycomb-Associated Non-Coding RNAs.
LF344735 - JP 2014500723-A/152238: Polycomb-Associated Non-Coding RNAs.
LF344734 - JP 2014500723-A/152237: Polycomb-Associated Non-Coding RNAs.
CU675359 - Synthetic construct Homo sapiens gateway clone IMAGE:100019098 5' read WDR74 mRNA.
LF344732 - JP 2014500723-A/152235: Polycomb-Associated Non-Coding RNAs.
AK303186 - Homo sapiens cDNA FLJ57690 complete cds, highly similar to WD repeat protein 74.
LF344731 - JP 2014500723-A/152234: Polycomb-Associated Non-Coding RNAs.
LF344729 - JP 2014500723-A/152232: Polycomb-Associated Non-Coding RNAs.
LF344726 - JP 2014500723-A/152229: Polycomb-Associated Non-Coding RNAs.
LF344725 - JP 2014500723-A/152228: Polycomb-Associated Non-Coding RNAs.
JD171047 - Sequence 152071 from Patent EP1572962.
MA580316 - JP 2018138019-A/152242: Polycomb-Associated Non-Coding RNAs.
MA580315 - JP 2018138019-A/152241: Polycomb-Associated Non-Coding RNAs.
MA580313 - JP 2018138019-A/152239: Polycomb-Associated Non-Coding RNAs.
MA580312 - JP 2018138019-A/152238: Polycomb-Associated Non-Coding RNAs.
MA580311 - JP 2018138019-A/152237: Polycomb-Associated Non-Coding RNAs.
MA580309 - JP 2018138019-A/152235: Polycomb-Associated Non-Coding RNAs.
MA580308 - JP 2018138019-A/152234: Polycomb-Associated Non-Coding RNAs.
MA580306 - JP 2018138019-A/152232: Polycomb-Associated Non-Coding RNAs.
MA580303 - JP 2018138019-A/152229: Polycomb-Associated Non-Coding RNAs.
MA580302 - JP 2018138019-A/152228: Polycomb-Associated Non-Coding RNAs.
MA446803 - JP 2018138019-A/18729: Polycomb-Associated Non-Coding RNAs.

-  Other Names for This Gene
  Alternate Gene Symbols: A8K8G5, ENST00000278856.1, ENST00000278856.2, ENST00000278856.3, ENST00000278856.4, ENST00000278856.5, ENST00000278856.6, ENST00000278856.7, ENST00000278856.8, NR_161381, NSA1, Q6RFH5, Q9BRC9, Q9H6X8, Q9NVY2, uc317jqb.1, uc317jqb.2, WDR74_HUMAN
UCSC ID: ENST00000278856.9_8
RefSeq Accession: NM_001369450.1
Protein: Q6RFH5 (aka WDR74_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.