Human Gene WLS (ENST00000262348.9_7) from GENCODE V47lift37
  Description: Wnt ligand secretion mediator, transcript variant 1 (from RefSeq NM_024911.7)
Gencode Transcript: ENST00000262348.9_7
Gencode Gene: ENSG00000116729.14_14
Transcript (Including UTRs)
   Position: hg19 chr1:68,591,043-68,698,206 Size: 107,164 Total Exon Count: 12 Strand: -
Coding Region
   Position: hg19 chr1:68,591,909-68,697,982 Size: 106,074 Coding Exon Count: 12 

Page IndexSequence and LinksUniProtKB CommentsPrimersMalaCardsCTD
Gene AllelesRNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther Species
GO AnnotationsmRNA DescriptionsPathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr1:68,591,043-68,698,206)mRNA (may differ from genome)Protein (541 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
AlphaFoldBioGPSEnsemblEntrez GeneExonPrimerGeneCards
HGNCMalacardsMGIOMIMPubMedReactome
UniProtKBWikipediaBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: WLS_HUMAN
DESCRIPTION: RecName: Full=Protein wntless homolog; AltName: Full=Integral membrane protein GPR177; AltName: Full=Protein evenness interrupted homolog; Short=EVI; AltName: Full=Putative NF-kappa-B-activating protein 373; Flags: Precursor;
FUNCTION: Regulates Wnt proteins sorting and secretion in a feedback regulatory mechanism. This reciprocal interaction plays a key role in the regulation of expression, subcellular location, binding and organelle-specific association of Wnt proteins. Plays also an important role in establishment of the anterior-posterior body axis formation during development (By similarity).
SUBUNIT: Interacts with WNT3A. Interacts with WNT1, WNT3 and WNT5A (By similarity).
INTERACTION: P35372:OPRM1; NbExp=7; IntAct=EBI-2868748, EBI-2624570;
SUBCELLULAR LOCATION: Golgi apparatus membrane; Multi-pass membrane protein. Cytoplasmic vesicle membrane; Multi-pass membrane protein.
SIMILARITY: Belongs to the wntless family.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  MalaCards Disease Associations
  MalaCards Gene Search: WLS
Diseases sorted by gene-association score: chromosome 1p32-p31 deletion syndrome (3), chronic intestinal vascular insufficiency (2)

-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 48.64 RPKM in Esophagus - Gastroesophageal Junction
Total median expression: 826.72 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -89.90224-0.401 Picture PostScript Text
3' UTR -237.10866-0.274 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR009551 - wntless

Pfam Domains:
PF06664 - Wnt-binding factor required for Wnt secretion

ModBase Predicted Comparative 3D Structure on Q5T9L3
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologGenome BrowserNo ortholog
Gene DetailsGene Details Gene DetailsGene Details 
Gene SorterGene Sorter Gene SorterGene Sorter 
 RGDEnsembl WormBase 
    Protein Sequence 
    Alignment 

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0005515 protein binding
GO:0017147 Wnt-protein binding
GO:0031852 mu-type opioid receptor binding

Biological Process:
GO:0001707 mesoderm formation
GO:0006886 intracellular protein transport
GO:0007275 multicellular organism development
GO:0009948 anterior/posterior axis specification
GO:0016055 Wnt signaling pathway
GO:0030177 positive regulation of Wnt signaling pathway
GO:0030901 midbrain development
GO:0030902 hindbrain development
GO:0031017 exocrine pancreas development
GO:0043123 positive regulation of I-kappaB kinase/NF-kappaB signaling
GO:0061355 Wnt protein secretion
GO:0061357 positive regulation of Wnt protein secretion
GO:0090263 positive regulation of canonical Wnt signaling pathway

Cellular Component:
GO:0000139 Golgi membrane
GO:0005768 endosome
GO:0005769 early endosome
GO:0005783 endoplasmic reticulum
GO:0005789 endoplasmic reticulum membrane
GO:0005794 Golgi apparatus
GO:0005802 trans-Golgi network
GO:0005886 plasma membrane
GO:0016020 membrane
GO:0016021 integral component of membrane
GO:0030659 cytoplasmic vesicle membrane
GO:0030666 endocytic vesicle membrane
GO:0031410 cytoplasmic vesicle
GO:0031901 early endosome membrane
GO:0032590 dendrite membrane
GO:0032839 dendrite cytoplasm
GO:0070062 extracellular exosome


-  Descriptions from all associated GenBank mRNAs
  AK301613 - Homo sapiens cDNA FLJ54765 complete cds.
BX538320 - Homo sapiens mRNA; cDNA DKFZp686K06169 (from clone DKFZp686K06169).
DQ323735 - Homo sapiens wntless protein (WLS) mRNA, complete cds.
AK074984 - Homo sapiens cDNA FLJ90503 fis, clone NT2RP3004083, highly similar to Putative NFkB activating protein 373.
AY359035 - Homo sapiens clone DNA44174 AGAI857 (UNQ857) mRNA, complete cds.
BC110826 - Homo sapiens G protein-coupled receptor 177, mRNA (cDNA clone MGC:131760 IMAGE:6183768), complete cds.
BX648748 - Homo sapiens mRNA; cDNA DKFZp686G1457 (from clone DKFZp686G1457).
AK074583 - Homo sapiens cDNA FLJ90102 fis, clone HEMBA1006299, highly similar to Putative NFkB activating protein 373.
BC137109 - Homo sapiens G protein-coupled receptor 177, mRNA (cDNA clone MGC:168729 IMAGE:9021106), complete cds.
BC137113 - Homo sapiens G protein-coupled receptor 177, mRNA (cDNA clone MGC:168733 IMAGE:9021110), complete cds.
AK309779 - Homo sapiens cDNA, FLJ99820.
AB097018 - Homo sapiens mRNA for putative NFkB activating protein, complete cds, clone: 373.
AK026744 - Homo sapiens cDNA: FLJ23091 fis, clone LNG07220.
BC007211 - Homo sapiens G protein-coupled receptor 177, mRNA (cDNA clone IMAGE:3349207), partial cds.
AK074990 - Homo sapiens cDNA FLJ90509 fis, clone NT2RP3004294, weakly similar to Xenopus laevis ER1 mRNA.
JD244345 - Sequence 225369 from Patent EP1572962.
JD357824 - Sequence 338848 from Patent EP1572962.
JD546788 - Sequence 527812 from Patent EP1572962.
JD066946 - Sequence 47970 from Patent EP1572962.
JD220640 - Sequence 201664 from Patent EP1572962.
JD461552 - Sequence 442576 from Patent EP1572962.
JD336504 - Sequence 317528 from Patent EP1572962.
JD106355 - Sequence 87379 from Patent EP1572962.
JD471912 - Sequence 452936 from Patent EP1572962.
JD340210 - Sequence 321234 from Patent EP1572962.
JD044476 - Sequence 25500 from Patent EP1572962.
JD361802 - Sequence 342826 from Patent EP1572962.
KJ903257 - Synthetic construct Homo sapiens clone ccsbBroadEn_12651 WLS gene, encodes complete protein.
CU675559 - Synthetic construct Homo sapiens gateway clone IMAGE:100019417 5' read GPR177 mRNA.
JD076225 - Sequence 57249 from Patent EP1572962.
JD381955 - Sequence 362979 from Patent EP1572962.
JD143209 - Sequence 124233 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q5T9L3 (Reactome details) participates in the following event(s):

R-HSA-3247839 Retromer associates with WLS
R-HSA-3247843 secretion of WNT ligands
R-HSA-3247840 WLS binds WNT ligands in the Golgi
R-HSA-3238698 WNT ligand biogenesis and trafficking
R-HSA-195721 Signaling by WNT
R-HSA-162582 Signal Transduction

-  Other Names for This Gene
  Alternate Gene Symbols: B2RNT2, C1orf139, ENST00000262348.1, ENST00000262348.2, ENST00000262348.3, ENST00000262348.4, ENST00000262348.5, ENST00000262348.6, ENST00000262348.7, ENST00000262348.8, GPR177, NM_024911, Q5JRS7, Q5T9L3, Q7Z2Z9, Q8NC43, uc317gzh.1, uc317gzh.2, UNQ85/PRO18667, WLS_HUMAN
UCSC ID: ENST00000262348.9_7
RefSeq Accession: NM_024911.7
Protein: Q5T9L3 (aka WLS_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.