Human Gene XPNPEP1 (ENST00000502935.6_7) from GENCODE V47lift37
  Description: X-prolyl aminopeptidase 1, transcript variant 1 (from RefSeq NM_020383.4)
Gencode Transcript: ENST00000502935.6_7
Gencode Gene: ENSG00000108039.18_9
Transcript (Including UTRs)
   Position: hg19 chr10:111,624,524-111,683,269 Size: 58,746 Total Exon Count: 21 Strand: -
Coding Region
   Position: hg19 chr10:111,624,942-111,683,191 Size: 58,250 Coding Exon Count: 21 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionMicroarray ExpressionRNA StructureProtein StructureOther SpeciesGO Annotations
mRNA DescriptionsOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr10:111,624,524-111,683,269)mRNA (may differ from genome)Protein (666 aa)
Gene SorterGenome BrowserOther Species FASTAVisiGeneGene interactionsTable Schema
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BioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: XPP1_HUMAN
DESCRIPTION: RecName: Full=Xaa-Pro aminopeptidase 1; EC=3.4.11.9; AltName: Full=Aminoacylproline aminopeptidase; AltName: Full=Cytosolic aminopeptidase P; AltName: Full=Soluble aminopeptidase P; Short=sAmp; AltName: Full=X-Pro aminopeptidase 1; AltName: Full=X-prolyl aminopeptidase 1, soluble;
FUNCTION: Contributes to the degradation of bradykinin. Catalyzes the removal of a penultimate prolyl residue from the N-termini of peptides, such as Arg-Pro-Pro.
CATALYTIC ACTIVITY: Release of any N-terminal amino acid, including proline, that is linked to proline, even from a dipeptide or tripeptide.
COFACTOR: Binds 2 manganese ions per subunit.
ENZYME REGULATION: Inhibited by apstatin and the metal ion chelators EDTA and 1,10-phenanthroline. Partially inhibited by dithiothreitol. Not inhibited by enalaprilat or amastatin.
BIOPHYSICOCHEMICAL PROPERTIES: Kinetic parameters: KM=100.6 uM for bradykinin; KM=308 uM for the tripeptide Arg-Pro-Pro; pH dependence: Optimum pH is 8.2;
SUBUNIT: Homodimer.
SUBCELLULAR LOCATION: Cytoplasm (By similarity).
TISSUE SPECIFICITY: Expressed in all tissues tested, including pancreas, heart, muscle, kidney, liver, lung and brain. Highest levels in pancreas.
SIMILARITY: Belongs to the peptidase M24B family.
SEQUENCE CAUTION: Sequence=CAD38640.1; Type=Erroneous initiation;

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 25.74 RPKM in Small Intestine - Terminal Ileum
Total median expression: 696.66 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

+  Microarray Expression Data
  Press "+" in the title bar above to open this section.

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -30.1078-0.386 Picture PostScript Text
3' UTR -96.20418-0.230 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR000587 - Creatinase
IPR000994 - Pept_M24_structural-domain
IPR001131 - Peptidase_M24B_aminopep-P_CS

Pfam Domains:
PF00557 - Metallopeptidase family M24
PF01321 - Creatinase/Prolidase N-terminal domain
PF16188 - C-terminal region of peptidase_M24
PF16189 - Creatinase/Prolidase N-terminal domain

SCOP Domains:
53092 - Creatinase/prolidase N-terminal domain
55920 - Creatinase/aminopeptidase

Protein Data Bank (PDB) 3-D Structure
MuPIT help
3CTZ - X-ray MuPIT


ModBase Predicted Comparative 3D Structure on Q9NQW7
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologGenome BrowserGenome Browser
Gene DetailsGene Details Gene DetailsGene DetailsGene Details
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 RGDEnsembl WormBaseSGD
    Protein SequenceProtein Sequence
    AlignmentAlignment

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0004177 aminopeptidase activity
GO:0008233 peptidase activity
GO:0008237 metallopeptidase activity
GO:0016787 hydrolase activity
GO:0030145 manganese ion binding
GO:0042803 protein homodimerization activity
GO:0046872 metal ion binding
GO:0070006 metalloaminopeptidase activity

Biological Process:
GO:0006508 proteolysis
GO:0010815 bradykinin catabolic process

Cellular Component:
GO:0005737 cytoplasm
GO:0005829 cytosol
GO:0070062 extracellular exosome


-  Descriptions from all associated GenBank mRNAs
  BC005126 - Homo sapiens X-prolyl aminopeptidase (aminopeptidase P) 1, soluble, mRNA (cDNA clone MGC:10592 IMAGE:3836436), complete cds.
AF195530 - Homo sapiens soluble aminopeptidase P (XPNPEP1) mRNA, complete cds.
BX537682 - Homo sapiens mRNA; cDNA DKFZp686I06222 (from clone DKFZp686I06222).
BC007579 - Homo sapiens X-prolyl aminopeptidase (aminopeptidase P) 1, soluble, mRNA (cDNA clone MGC:15561 IMAGE:3139868), complete cds.
AK093693 - Homo sapiens cDNA FLJ36374 fis, clone THYMU2008185, highly similar to Xaa-Pro aminopeptidase 1 (EC 3.4.11.9).
AL833411 - Homo sapiens mRNA; cDNA DKFZp313D1022 (from clone DKFZp313D1022).
AK289436 - Homo sapiens cDNA FLJ76203 complete cds, highly similar to Homo sapiens X-prolyl aminopeptidase (aminopeptidase P) 1, soluble (XPNPEP1), mRNA.
AK223513 - Homo sapiens mRNA for X-prolyl aminopeptidase (aminopeptidase P) 1, soluble variant, clone: FCC122C02.
AK304367 - Homo sapiens cDNA FLJ58474 complete cds, highly similar to Xaa-Pro aminopeptidase 1 (EC 3.4.11.9).
AK095447 - Homo sapiens cDNA FLJ38128 fis, clone D6OST2000358, highly similar to H.sapiens mRNA for aminopeptidase P-like.
AK094900 - Homo sapiens cDNA FLJ37581 fis, clone BRCOC2004175.
BC013417 - Homo sapiens X-prolyl aminopeptidase (aminopeptidase P) 1, soluble, mRNA (cDNA clone IMAGE:3546113), partial cds.
JD245121 - Sequence 226145 from Patent EP1572962.
JD079102 - Sequence 60126 from Patent EP1572962.
JD403816 - Sequence 384840 from Patent EP1572962.
JD391451 - Sequence 372475 from Patent EP1572962.
JD262727 - Sequence 243751 from Patent EP1572962.
JD086133 - Sequence 67157 from Patent EP1572962.
AF272981 - Homo sapiens cytosolic aminopeptidase P mRNA, complete cds.
CR456922 - Homo sapiens full open reading frame cDNA clone RZPDo834D1221D for gene XPNPEP1, X-prolyl aminopeptidase (aminopeptidase P) 1, soluble; complete cds, incl. stopcodon.
X95762 - H.sapiens mRNA for aminopeptidase P-like.
EU176414 - Synthetic construct Homo sapiens clone IMAGE:100006627; FLH264103.01X; RZPDo839D12257D X-prolyl aminopeptidase (aminopeptidase P) 1, soluble (XPNPEP1) gene, encodes complete protein.
KJ901828 - Synthetic construct Homo sapiens clone ccsbBroadEn_11222 XPNPEP1 gene, encodes complete protein.
EU176811 - Synthetic construct Homo sapiens clone IMAGE:100011631; FLH263980.01L; RZPDo839C04245D X-prolyl aminopeptidase (aminopeptidase P) 1, soluble (XPNPEP1) gene, encodes complete protein.
AK295756 - Homo sapiens cDNA FLJ57731 complete cds, highly similar to Xaa-Pro aminopeptidase 1 (EC 3.4.11.9).
CU675715 - Synthetic construct Homo sapiens gateway clone IMAGE:100023481 5' read XPNPEP1 mRNA.
JD025334 - Sequence 6358 from Patent EP1572962.
JD024450 - Sequence 5474 from Patent EP1572962.
JD035225 - Sequence 16249 from Patent EP1572962.
JD034892 - Sequence 15916 from Patent EP1572962.
JD056853 - Sequence 37877 from Patent EP1572962.

-  Other Names for This Gene
  Alternate Gene Symbols: A8K071, ENST00000502935.1, ENST00000502935.2, ENST00000502935.3, ENST00000502935.4, ENST00000502935.5, G5E9Y2, G8JLB2, NM_020383, O15250, Q53EX6, Q8N3Q0, Q96D23, Q9NQW7, uc322wnp.1, uc322wnp.2, XPNPEP1 , XPNPEPL , XPNPEPL1, XPP1_HUMAN
UCSC ID: ENST00000502935.6_7
RefSeq Accession: NM_020383.4
Protein: Q9NQW7 (aka XPP1_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.