Human Gene ZHX1 (ENST00000395571.8_7) from GENCODE V47lift37
  Description: zinc fingers and homeoboxes 1, transcript variant 2 (from RefSeq NM_007222.5)
Gencode Transcript: ENST00000395571.8_7
Gencode Gene: ENSG00000165156.15_11
Transcript (Including UTRs)
   Position: hg19 chr8:124,260,696-124,286,526 Size: 25,831 Total Exon Count: 4 Strand: -
Coding Region
   Position: hg19 chr8:124,265,565-124,268,186 Size: 2,622 Coding Exon Count: 1 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
Other NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr8:124,260,696-124,286,526)mRNA (may differ from genome)Protein (873 aa)
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BioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: ZHX1_HUMAN
DESCRIPTION: RecName: Full=Zinc fingers and homeoboxes protein 1;
FUNCTION: Acts as a transcriptional repressor. Increases DNMT3B- mediated repressive transcriptional activity when DNMT3B is tethered to DNA. May link molecule between DNMT3B and other co- repressor proteins.
SUBUNIT: Forms homodimers. Heterodimer (via HD1 domain) with ZHX2 (via HD1 domain). Also forms heterodimer with ZHX3 which is a prerequisite for repressor activity. Interacts with ATF7IP and NFYA. Interacts (via homeobox domains) with DNMT3B (via PWWP domain).
INTERACTION: P54253:ATXN1; NbExp=5; IntAct=EBI-347767, EBI-930964; Q9UBC3-1:DNMT3B; NbExp=4; IntAct=EBI-347767, EBI-6083193;
SUBCELLULAR LOCATION: Nucleus. Note=Colocalized in the nucleus with DNMT3B.
TISSUE SPECIFICITY: Ubiquitously expressed. Expressed in podocytes.
PTM: Phosphorylated upon DNA damage, probably by ATM or ATR.
SIMILARITY: Belongs to the ZHX family.
SIMILARITY: Contains 2 C2H2-type zinc fingers.
SIMILARITY: Contains 5 homeobox DNA-binding domains.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene           more ... click here to view the complete list

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 10.86 RPKM in Esophagus - Mucosa
Total median expression: 324.70 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -111.20409-0.272 Picture PostScript Text
3' UTR -391.101868-0.209 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR001356 - Homeodomain
IPR009057 - Homeodomain-like
IPR007087 - Znf_C2H2
IPR015880 - Znf_C2H2-like

Pfam Domains:
PF00046 - Homeodomain
PF05920 - Homeobox KN domain
PF11569 - Homeodomain leucine-zipper encoding, Homez
PF18387 - Zinc-fingers and homeoboxes C2H2 finger domain

SCOP Domains:
46689 - Homeodomain-like
57667 - beta-beta-alpha zinc fingers
118359 - Expressed protein At2g23090/F21P24.15

Protein Data Bank (PDB) 3-D Structure
MuPIT help
2ECB - NMR MuPIT 2GHF - NMR MuPIT 3NAR - X-ray MuPIT


ModBase Predicted Comparative 3D Structure on Q9UKY1
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The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
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-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000981 RNA polymerase II transcription factor activity, sequence-specific DNA binding
GO:0003676 nucleic acid binding
GO:0003677 DNA binding
GO:0003700 transcription factor activity, sequence-specific DNA binding
GO:0003714 transcription corepressor activity
GO:0005515 protein binding
GO:0046872 metal ion binding
GO:0046982 protein heterodimerization activity

Biological Process:
GO:0000122 negative regulation of transcription from RNA polymerase II promoter
GO:0006351 transcription, DNA-templated
GO:0006355 regulation of transcription, DNA-templated
GO:0030154 cell differentiation
GO:0045892 negative regulation of transcription, DNA-templated

Cellular Component:
GO:0005634 nucleus
GO:0005654 nucleoplasm


-  Descriptions from all associated GenBank mRNAs
  BC012379 - Homo sapiens chromosome 8 open reading frame 76, mRNA (cDNA clone MGC:9784 IMAGE:3857253), complete cds.
KJ903490 - Synthetic construct Homo sapiens clone ccsbBroadEn_12884 C8orf76 gene, encodes complete protein.
AK025236 - Homo sapiens cDNA: FLJ21583 fis, clone COL06826, highly similar to AF195766 Homo sapiens ZHX1 protein (ZHX1) mRNA.
BC040481 - Homo sapiens zinc fingers and homeoboxes 1, mRNA (cDNA clone MGC:33068 IMAGE:5271344), complete cds.
JD265081 - Sequence 246105 from Patent EP1572962.
JD353392 - Sequence 334416 from Patent EP1572962.
JD244402 - Sequence 225426 from Patent EP1572962.
JD550927 - Sequence 531951 from Patent EP1572962.
AF106862 - Homo sapiens zinc finger homeobox protein ZHX1 mRNA, complete cds.
AK094451 - Homo sapiens cDNA FLJ37132 fis, clone BRACE2023223.
AF195766 - Homo sapiens ZHX1 protein (ZHX1) mRNA, complete cds.
JD285306 - Sequence 266330 from Patent EP1572962.
JD382840 - Sequence 363864 from Patent EP1572962.
JD184512 - Sequence 165536 from Patent EP1572962.
JD276768 - Sequence 257792 from Patent EP1572962.
JD549369 - Sequence 530393 from Patent EP1572962.
JD301984 - Sequence 283008 from Patent EP1572962.
JD091381 - Sequence 72405 from Patent EP1572962.
JD156319 - Sequence 137343 from Patent EP1572962.
JD441343 - Sequence 422367 from Patent EP1572962.
AK289532 - Homo sapiens cDNA FLJ78425 complete cds, highly similar to Homo sapiens zinc fingers and homeoboxes 1 (ZHX1), mRNA.
JD042477 - Sequence 23501 from Patent EP1572962.
AB489156 - Synthetic construct DNA, clone: pF1KB3240, Homo sapiens ZHX1 gene for zinc fingers and homeoboxes 1, without stop codon, in Flexi system.
JD061587 - Sequence 42611 from Patent EP1572962.
JD174580 - Sequence 155604 from Patent EP1572962.
JD452650 - Sequence 433674 from Patent EP1572962.
JD462511 - Sequence 443535 from Patent EP1572962.

-  Other Names for This Gene
  Alternate Gene Symbols: ENST00000395571.1, ENST00000395571.2, ENST00000395571.3, ENST00000395571.4, ENST00000395571.5, ENST00000395571.6, ENST00000395571.7, NM_007222, Q8IWD8, Q9UKY1, uc318xna.1, uc318xna.2, ZHX1_HUMAN
UCSC ID: ENST00000395571.8_7
RefSeq Accession: NM_007222.5
Protein: Q9UKY1 (aka ZHX1_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.