Human Gene ZNF514 (ENST00000295208.7_8) from GENCODE V47lift37
  Description: zinc finger protein 514, transcript variant 2 (from RefSeq NM_032788.3)
Gencode Transcript: ENST00000295208.7_8
Gencode Gene: ENSG00000144026.14_15
Transcript (Including UTRs)
   Position: hg19 chr2:95,810,745-95,825,593 Size: 14,849 Total Exon Count: 5 Strand: -
Coding Region
   Position: hg19 chr2:95,815,027-95,818,998 Size: 3,972 Coding Exon Count: 3 

Page IndexSequence and LinksUniProtKB CommentsPrimersCTDGene Alleles
RNA-Seq ExpressionRNA StructureProtein StructureOther SpeciesGO AnnotationsmRNA Descriptions
PathwaysOther NamesModel InformationMethods
Data last updated at UCSC: 2024-08-22 23:36:26

-  Sequence and Links to Tools and Databases
 
Genomic Sequence (chr2:95,810,745-95,825,593)mRNA (may differ from genome)Protein (400 aa)
Gene SorterGenome BrowserOther Species FASTAGene interactionsTable SchemaAlphaFold
BioGPSEnsemblEntrez GeneExonPrimerGeneCardsHGNC
MGIPubMedReactomeUniProtKBBioGrid CRISPR DB

-  Comments and Description Text from UniProtKB
  ID: ZN514_HUMAN
DESCRIPTION: RecName: Full=Zinc finger protein 514;
FUNCTION: May be involved in transcriptional regulation.
SUBCELLULAR LOCATION: Nucleus (Potential).
SIMILARITY: Belongs to the krueppel C2H2-type zinc-finger protein family.
SIMILARITY: Contains 7 C2H2-type zinc fingers.
SIMILARITY: Contains 1 KRAB domain.

-  Primer design for this transcript
 

Primer3Plus can design qPCR Primers that straddle exon-exon-junctions, which amplify only cDNA, not genomic DNA.
Click here to load the transcript sequence and exon structure into Primer3Plus

Exonprimer can design one pair of Sanger sequencing primers around every exon, located in non-genic sequence.
Click here to open Exonprimer with this transcript

To design primers for a non-coding sequence, zoom to a region of interest and select from the drop-down menu: View > In External Tools > Primer3


-  Comparative Toxicogenomics Database (CTD)
  The following chemicals interact with this gene

+  Common Gene Haplotype Alleles
  Press "+" in the title bar above to open this section.

-  RNA-Seq Expression Data from GTEx (53 Tissues, 570 Donors)
  Highest median expression: 9.80 RPKM in Ovary
Total median expression: 204.15 RPKM



View in GTEx track of Genome Browser    View at GTEx portal     View GTEx Body Map

-  mRNA Secondary Structure of 3' and 5' UTRs
 
RegionFold EnergyBasesEnergy/Base
Display As
5' UTR -397.70704-0.565 Picture PostScript Text
3' UTR -1392.904282-0.325 Picture PostScript Text

The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.

-  Protein Domain and Structure Information
  InterPro Domains: Graphical view of domain structure
IPR001909 - Krueppel-associated_box
IPR007087 - Znf_C2H2
IPR015880 - Znf_C2H2-like
IPR013087 - Znf_C2H2/integrase_DNA-bd

Pfam Domains:
PF00096 - Zinc finger, C2H2 type
PF01352 - KRAB box
PF13912 - C2H2-type zinc finger
PF13913 - zinc-finger of a C2HC-type

SCOP Domains:
109640 - KRAB domain (Kruppel-associated box)
55550 - SH2 domain
57667 - beta-beta-alpha zinc fingers
57716 - Glucocorticoid receptor-like (DNA-binding domain)
57774 - Microbial and mitochondrial ADK, insert "zinc finger" domain
57783 - Zinc beta-ribbon
57802 - Rubredoxin-like
57903 - FYVE/PHD zinc finger

ModBase Predicted Comparative 3D Structure on Q96K75
FrontTopSide
The pictures above may be empty if there is no ModBase structure for the protein. The ModBase structure frequently covers just a fragment of the protein. You may be asked to log onto ModBase the first time you click on the pictures. It is simplest after logging in to just click on the picture again to get to the specific info on that model.

-  Orthologous Genes in Other Species
  Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
MouseRatZebrafishD. melanogasterC. elegansS. cerevisiae
No orthologNo orthologNo orthologNo orthologNo orthologNo ortholog
      
      
      
      
      

-  Gene Ontology (GO) Annotations with Structured Vocabulary
  Molecular Function:
GO:0000981 RNA polymerase II transcription factor activity, sequence-specific DNA binding
GO:0003676 nucleic acid binding
GO:0003677 DNA binding
GO:0003700 transcription factor activity, sequence-specific DNA binding
GO:0046872 metal ion binding

Biological Process:
GO:0006351 transcription, DNA-templated
GO:0006355 regulation of transcription, DNA-templated
GO:0006357 regulation of transcription from RNA polymerase II promoter

Cellular Component:
GO:0005622 intracellular
GO:0005634 nucleus


-  Descriptions from all associated GenBank mRNAs
  AL833754 - Homo sapiens mRNA; cDNA DKFZp666C237 (from clone DKFZp666C237).
AL832263 - Homo sapiens mRNA; cDNA DKFZp667J1717 (from clone DKFZp667J1717).
BC068498 - Homo sapiens zinc finger protein 514, mRNA (cDNA clone IMAGE:30344612), with apparent retained intron.
AK027363 - Homo sapiens cDNA FLJ14457 fis, clone HEMBB1002217, weakly similar to ZINC FINGER PROTEIN 91.
JD101955 - Sequence 82979 from Patent EP1572962.
JD481409 - Sequence 462433 from Patent EP1572962.
JD370235 - Sequence 351259 from Patent EP1572962.
JD073051 - Sequence 54075 from Patent EP1572962.
JD444765 - Sequence 425789 from Patent EP1572962.
JD074680 - Sequence 55704 from Patent EP1572962.
JD257186 - Sequence 238210 from Patent EP1572962.
JD351927 - Sequence 332951 from Patent EP1572962.
JD048030 - Sequence 29054 from Patent EP1572962.
JD171645 - Sequence 152669 from Patent EP1572962.
JD143735 - Sequence 124759 from Patent EP1572962.
JD171644 - Sequence 152668 from Patent EP1572962.
JD440897 - Sequence 421921 from Patent EP1572962.
JD432066 - Sequence 413090 from Patent EP1572962.
JD106638 - Sequence 87662 from Patent EP1572962.
JD106637 - Sequence 87661 from Patent EP1572962.
JD357935 - Sequence 338959 from Patent EP1572962.
JD079600 - Sequence 60624 from Patent EP1572962.
JD244663 - Sequence 225687 from Patent EP1572962.
JD243237 - Sequence 224261 from Patent EP1572962.
JD136207 - Sequence 117231 from Patent EP1572962.
JD246821 - Sequence 227845 from Patent EP1572962.
JD413327 - Sequence 394351 from Patent EP1572962.
JD198037 - Sequence 179061 from Patent EP1572962.
JD212660 - Sequence 193684 from Patent EP1572962.
JD542697 - Sequence 523721 from Patent EP1572962.
JD246820 - Sequence 227844 from Patent EP1572962.
JD246818 - Sequence 227842 from Patent EP1572962.
JD188056 - Sequence 169080 from Patent EP1572962.
JD461909 - Sequence 442933 from Patent EP1572962.
JD314658 - Sequence 295682 from Patent EP1572962.
JD037129 - Sequence 18153 from Patent EP1572962.
JD237817 - Sequence 218841 from Patent EP1572962.
JD353743 - Sequence 334767 from Patent EP1572962.
JD438221 - Sequence 419245 from Patent EP1572962.
JD232023 - Sequence 213047 from Patent EP1572962.
JD256220 - Sequence 237244 from Patent EP1572962.
JD410583 - Sequence 391607 from Patent EP1572962.
JD269068 - Sequence 250092 from Patent EP1572962.
BC110524 - Homo sapiens zinc finger protein 514, mRNA (cDNA clone MGC:126229 IMAGE:40034154), complete cds.
BC110525 - Homo sapiens zinc finger protein 514, mRNA (cDNA clone MGC:126230 IMAGE:40034155), complete cds.
JD548407 - Sequence 529431 from Patent EP1572962.
JD434467 - Sequence 415491 from Patent EP1572962.
JD071335 - Sequence 52359 from Patent EP1572962.
JD076866 - Sequence 57890 from Patent EP1572962.
JD044997 - Sequence 26021 from Patent EP1572962.
JD236671 - Sequence 217695 from Patent EP1572962.
JD065693 - Sequence 46717 from Patent EP1572962.
JD146487 - Sequence 127511 from Patent EP1572962.
JD297109 - Sequence 278133 from Patent EP1572962.
BC074900 - Homo sapiens zinc finger protein 514, mRNA (cDNA clone MGC:104036 IMAGE:30915480), complete cds.
BC074901 - Homo sapiens zinc finger protein 514, mRNA (cDNA clone MGC:103869 IMAGE:30915251), complete cds.
KJ895044 - Synthetic construct Homo sapiens clone ccsbBroadEn_04438 ZNF514 gene, encodes complete protein.
AB463369 - Synthetic construct DNA, clone: pF1KB8736, Homo sapiens ZNF514 gene for zinc finger protein 514, without stop codon, in Flexi system.
JD314878 - Sequence 295902 from Patent EP1572962.
AF024697 - Homo sapiens clone L3-3 zinc finger protein mRNA, partial cds.
JD357941 - Sequence 338965 from Patent EP1572962.
JD414008 - Sequence 395032 from Patent EP1572962.
JD391312 - Sequence 372336 from Patent EP1572962.
JD180691 - Sequence 161715 from Patent EP1572962.
JD267199 - Sequence 248223 from Patent EP1572962.
JD066486 - Sequence 47510 from Patent EP1572962.
JD215455 - Sequence 196479 from Patent EP1572962.

-  Biochemical and Signaling Pathways
  Reactome (by CSHL, EBI, and GO)

Protein Q96K75 (Reactome details) participates in the following event(s):

R-HSA-975040 KRAB-ZNF / KAP Interaction
R-HSA-212436 Generic Transcription Pathway
R-HSA-73857 RNA Polymerase II Transcription
R-HSA-74160 Gene expression (Transcription)

-  Other Names for This Gene
  Alternate Gene Symbols: ENST00000295208.1, ENST00000295208.2, ENST00000295208.3, ENST00000295208.4, ENST00000295208.5, ENST00000295208.6, NM_032788, Q5JPJ3, Q96K75, uc317lkl.1, uc317lkl.2, ZN514_HUMAN
UCSC ID: ENST00000295208.7_8
RefSeq Accession: NM_032788.3
Protein: Q96K75 (aka ZN514_HUMAN or Z514_HUMAN)

-  Gene Model Information
  Click here for a detailed description of the fields of the table above.

-  Methods, Credits, and Use Restrictions
  Click here for details on how this gene model was made and data restrictions if any.