ID:ZW10_HUMAN DESCRIPTION: RecName: Full=Centromere/kinetochore protein zw10 homolog; FUNCTION: Essential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis. Required for the assembly of the dynein-dynactin and MAD1-MAD2 complexes onto kinetochores. Involved in regulation of membrane traffic between the Golgi and the endoplasmic reticulum. SUBUNIT: Associated with a SNARE complex consisting of STX18, USE1L, BNIP1/SEC20L, and SEC22B through direct interaction with RINT1/TIP20L bound to BNIP1/SEC20L. Component of the RZZ complex composed of KNTC1/ROD, ZW10 and ZWILCH. Interacts with C19orf25, KNTC1, and ZWINT. INTERACTION: O95229:ZWINT; NbExp=6; IntAct=EBI-1001217, EBI-1001132; SUBCELLULAR LOCATION: Cytoplasm. Endoplasmic reticulum membrane; Peripheral membrane protein. Chromosome, centromere, kinetochore. Cytoplasm, cytoskeleton, spindle. Note=Dynamic pattern of localization during the cell cycle. In most cells at interphase, present diffusely in the cytoplasm. In prometaphase, associated with the kinetochore. At metaphase, detected both at the kinetochores and, most prominently, at the spindle, particularly at the spindle poles. In very early anaphase, detected on segregating kinetochores. In late anaphase and telophase, accumulates at the spindle midzone. TISSUE SPECIFICITY: Widely expressed. DEVELOPMENTAL STAGE: No significant variation in expression during cell cycle. MISCELLANEOUS: Overexpression as well as silencing of ZW10 disrupts the morphology of the ER-Golgi intermediate compartment as well as the Golgi apparatus and slows down ER-Golgi transport. SIMILARITY: Belongs to the ZW10 family.
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on O43264
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
AK303871 - Homo sapiens cDNA FLJ56935 complete cds, highly similar to Centromere/kinetochore protein zw10 homolog. U54996 - Human protein ZW10 homolog (HZW10) mRNA, complete cds. BC128264 - Homo sapiens ZW10, kinetochore associated, homolog (Drosophila), mRNA (cDNA clone MGC:149821 IMAGE:40118818), complete cds. AK300746 - Homo sapiens cDNA FLJ56672 complete cds, highly similar to Centromere/kinetochore protein zw10 homolog. AK312925 - Homo sapiens cDNA, FLJ93375, highly similar to Homo sapiens ZW10, kinetochore associated, homolog (Drosophila) (ZW10), mRNA. JD338884 - Sequence 319908 from Patent EP1572962. JD220753 - Sequence 201777 from Patent EP1572962. JD563585 - Sequence 544609 from Patent EP1572962. JD044065 - Sequence 25089 from Patent EP1572962. JD194792 - Sequence 175816 from Patent EP1572962. DL492400 - Novel nucleic acids. DL490883 - Novel nucleic acids.
Biochemical and Signaling Pathways
Reactome (by CSHL, EBI, and GO)
Protein O43264 (Reactome details) participates in the following event(s):
R-HSA-6811423 Retrograde vesicle is tethered at the ER by the NRZ complex and t-SNAREs R-HSA-141409 Mad1 binds kinetochore R-HSA-375302 Kinetochore capture of astral microtubules R-HSA-5666129 CDC42:GTP recruits DIAPH2-2 to kinetochores R-HSA-5666169 Kinetochore capture of astral microtubules is positively regulated by CDC42:GTP:p-S196-DIAPH2-2 R-HSA-141431 MAD2 associates with the Mad1 kinetochore complex R-HSA-141439 Release of activated MAD2 from kinetochores R-HSA-2467811 Separation of sister chromatids R-HSA-2467809 ESPL1 (Separase) cleaves centromeric cohesin R-HSA-5666160 AURKB phosphorylates DIAPH2-2 at kinetochores R-HSA-141422 MAD2 converted to an inhibitory state via interaction with Mad1 R-HSA-1638821 PP2A-B56 dephosphorylates centromeric cohesin R-HSA-1638803 Phosphorylation of cohesin by PLK1 at centromeres R-HSA-2468287 CDK1 phosphorylates CDCA5 (Sororin) at centromeres R-HSA-6811434 COPI-dependent Golgi-to-ER retrograde traffic R-HSA-141444 Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal R-HSA-68877 Mitotic Prometaphase R-HSA-5663220 RHO GTPases Activate Formins R-HSA-8856688 Golgi-to-ER retrograde transport R-HSA-2500257 Resolution of Sister Chromatid Cohesion R-HSA-2467813 Separation of Sister Chromatids R-HSA-141424 Amplification of signal from the kinetochores R-HSA-68886 M Phase R-HSA-195258 RHO GTPase Effectors R-HSA-6811442 Intra-Golgi and retrograde Golgi-to-ER traffic R-HSA-68882 Mitotic Anaphase R-HSA-69618 Mitotic Spindle Checkpoint R-HSA-69278 Cell Cycle (Mitotic) R-HSA-194315 Signaling by Rho GTPases R-HSA-199991 Membrane Trafficking R-HSA-2555396 Mitotic Metaphase and Anaphase R-HSA-69620 Cell Cycle Checkpoints R-HSA-1640170 Cell Cycle R-HSA-162582 Signal Transduction R-HSA-5653656 Vesicle-mediated transport
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Sequence and Links to Tools and Databases 
Common Gene Haplotype Alleles 
