ID:ZWILC_HUMAN DESCRIPTION: RecName: Full=Protein zwilch homolog; Short=hZwilch; FUNCTION: Essential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis. Required for the assembly of the dynein-dynactin and MAD1-MAD2 complexes onto kinetochores. SUBUNIT: Component of the RZZ complex composed of KNTC1/ROD, ZW10 and ZWILCH. Interacts directly with KNTC1/ROD. Component of the NRZ complex composed of ZWILCH, KNTC1/ROD, RINT1, ZW10 and NBAS. SUBCELLULAR LOCATION: Chromosome, centromere, kinetochore. MISCELLANEOUS: ZWILCH gene is deleted in a patient suffering from colorectal cancer with chromosomal instability. SIMILARITY: Belongs to the ZWILCH family. SEQUENCE CAUTION: Sequence=AK000898; Type=Erroneous termination; Positions=366; Note=Translated as Leu; Sequence=AK000898; Type=Frameshift; Positions=248; Sequence=BAB55133.1; Type=Erroneous initiation;
The RNAfold program from the Vienna RNA Package is used to perform the secondary structure predictions and folding calculations. The estimated folding energy is in kcal/mol. The more negative the energy, the more secondary structure the RNA is likely to have.
ModBase Predicted Comparative 3D Structure on Q9H900
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Orthologous Genes in Other Species
Orthologies between human, mouse, and rat are computed by taking the best BLASTP hit, and filtering out non-syntenic hits. For more distant species reciprocal-best BLASTP hits are used. Note that the absence of an ortholog in the table below may reflect incomplete annotations in the other species rather than a true absence of the orthologous gene.
AK023175 - Homo sapiens cDNA FLJ13113 fis, clone NT2RP3002590. BX640701 - Homo sapiens mRNA; cDNA DKFZp686A05136 (from clone DKFZp686A05136). AK122787 - Homo sapiens cDNA FLJ16343 fis, clone TESTI2031529, highly similar to Homo sapiens Zwilch, kinetochore associated, homolog (Drosophila) (ZWILCH), mRNA. AK291048 - Homo sapiens cDNA FLJ77716 complete cds, highly similar to Homo sapiens Zwilch, kinetochore associated, homolog (Drosophila), mRNA. BC090041 - Homo sapiens Zwilch, kinetochore associated, homolog (Drosophila), mRNA (cDNA clone MGC:111034 IMAGE:6472990), complete cds. BC036900 - Homo sapiens Zwilch, kinetochore associated, homolog (Drosophila), mRNA (cDNA clone MGC:46079 IMAGE:5535106), complete cds. AK000898 - Homo sapiens cDNA FLJ10036 fis, clone HEMBA1000946. JD241247 - Sequence 222271 from Patent EP1572962. KJ902752 - Synthetic construct Homo sapiens clone ccsbBroadEn_12146 ZWILCH gene, encodes complete protein. JD564670 - Sequence 545694 from Patent EP1572962. AK027468 - Homo sapiens cDNA FLJ14562 fis, clone NT2RM4000027. JD378516 - Sequence 359540 from Patent EP1572962. JD449100 - Sequence 430124 from Patent EP1572962. JD304083 - Sequence 285107 from Patent EP1572962.
Biochemical and Signaling Pathways
Reactome (by CSHL, EBI, and GO)
Protein Q9H900 (Reactome details) participates in the following event(s):
R-HSA-141409 Mad1 binds kinetochore R-HSA-375302 Kinetochore capture of astral microtubules R-HSA-5666129 CDC42:GTP recruits DIAPH2-2 to kinetochores R-HSA-5666169 Kinetochore capture of astral microtubules is positively regulated by CDC42:GTP:p-S196-DIAPH2-2 R-HSA-141431 MAD2 associates with the Mad1 kinetochore complex R-HSA-141439 Release of activated MAD2 from kinetochores R-HSA-2467811 Separation of sister chromatids R-HSA-2467809 ESPL1 (Separase) cleaves centromeric cohesin R-HSA-5666160 AURKB phosphorylates DIAPH2-2 at kinetochores R-HSA-141422 MAD2 converted to an inhibitory state via interaction with Mad1 R-HSA-1638821 PP2A-B56 dephosphorylates centromeric cohesin R-HSA-1638803 Phosphorylation of cohesin by PLK1 at centromeres R-HSA-2468287 CDK1 phosphorylates CDCA5 (Sororin) at centromeres R-HSA-141444 Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal R-HSA-68877 Mitotic Prometaphase R-HSA-5663220 RHO GTPases Activate Formins R-HSA-2500257 Resolution of Sister Chromatid Cohesion R-HSA-2467813 Separation of Sister Chromatids R-HSA-141424 Amplification of signal from the kinetochores R-HSA-68886 M Phase R-HSA-195258 RHO GTPase Effectors R-HSA-68882 Mitotic Anaphase R-HSA-69618 Mitotic Spindle Checkpoint R-HSA-69278 Cell Cycle (Mitotic) R-HSA-194315 Signaling by Rho GTPases R-HSA-2555396 Mitotic Metaphase and Anaphase R-HSA-69620 Cell Cycle Checkpoints R-HSA-1640170 Cell Cycle R-HSA-162582 Signal Transduction
US Server
Sequence and Links to Tools and Databases 
Common Gene Haplotype Alleles 
