Cytokine 2001,
PMID: 11497497
Thabard, W; Collette, M; Mellerin, M P; Puthier, D; Barillé, S; Bataille, R; Amiot, M
Interleukin 6 (IL-6) is the major survival factor of myeloma cells. In this study, we demonstrate that IL-6, oncostatin M (OSM) and leukemia inhibitory factor (LIF) upregulate membrane IL-6 receptor alpha (IL-6Ralpha) on OPM-2 myeloma cell line at transcriptional level. In OPM-2 cells, IL-6, OSM and LIF induce both signal transducers and activators of transcription (STAT), mitogen activated protein kinase (MAPK) and phosphatidylinositol 3-kinase (PI 3-K) activation. We show that the cytokine-induced upregulation of IL-6Ralpha can be abolished by a janus kinase (JAK)-2 specific inhibitor, i.e. AG490, suggesting an involvement of the JAK/STAT pathway in this process. Finally, IL-6Ralpha upregulation was also inhibited by wortmannin, an inhibitor of the PI 3-kinase pathway. In conclusion, IL-6 can upregulate its own receptor on OPM-2 cells probably through the JAK/STAT and PI 3-kinase pathways.
Diseases/Pathways annotated by Medline MESH: MAP Kinase Signaling System, Multiple Myeloma
Document information provided by NCBI PubMed
Text Mining Data
MAPK → IL-6: "
In OPM-2 cells,
IL-6 , OSM and LIF
induce both signal transducers and activators of transcription ( STAT ), mitogen activated protein kinase (
MAPK ) and phosphatidylinositol 3-kinase (PI 3-K) activation
"
mitogen activated protein kinase → LIF: "
In OPM-2 cells, IL-6, OSM and LIF induce both signal transducers and activators of transcription ( STAT ), mitogen activated protein kinase ( MAPK ) and phosphatidylinositol 3-kinase (PI 3-K) activation
"
MAPK → OSM: "
In OPM-2 cells, IL-6, OSM and LIF induce both signal transducers and activators of transcription ( STAT ), mitogen activated protein kinase ( MAPK ) and phosphatidylinositol 3-kinase (PI 3-K) activation
"
IL-6 → phosphatidylinositol 3-kinase (PI 3-K): "
In OPM-2 cells, IL-6 , OSM and LIF induce both signal transducers and activators of transcription ( STAT ), mitogen activated protein kinase ( MAPK ) and phosphatidylinositol 3-kinase (PI 3-K) activation
"
LIF → phosphatidylinositol 3-kinase (PI 3-K): "
In OPM-2 cells, IL-6, OSM and LIF induce both signal transducers and activators of transcription ( STAT ), mitogen activated protein kinase ( MAPK ) and phosphatidylinositol 3-kinase (PI 3-K) activation
"
OSM → phosphatidylinositol 3-kinase (PI 3-K): "
In OPM-2 cells, IL-6, OSM and LIF induce both signal transducers and activators of transcription ( STAT ), mitogen activated protein kinase ( MAPK ) and phosphatidylinositol 3-kinase (PI 3-K) activation
"
Manually curated Databases
No curated data.