Gene interactions and pathways from curated databases and text-mining
Am J Physiol Lung Cell Mol Physiol 2001, PMID: 11557582

PDGF-BB-mediated activation of p42(MAPK) is independent of PDGF beta-receptor tyrosine phosphorylation.

Cartel, N J; Liu, J; Wang, J; Post, M

Herein, we investigated the activity of mitogen-activated protein kinase (MAPK), a key component of downstream signaling events, which is activated subsequent to platelet-derived growth factor (PDGF)-BB stimulation. Specifically, p42(MAPK) activity peaked 60 min after addition of PDGF-BB, declined thereafter, and was determined not to be a direct or necessary component of glycosaminoglycan (GAG) synthesis. PDGF-BB also activated MAPK kinase 2 (MAPKK2) but had no effect on MAPKK1 and Raf-1 activity. Chemical inhibition of Janus kinase, phosphatidylinositol 3-kinase, Src kinase, or tyrosine phosphorylation inhibition of the PDGF beta-receptor (PDGFR-beta) did not abrogate PDGF-BB-induced p42(MAPK) activation or its threonine or tyrosine phosphorylation. A dominant negative cytoplasmic receptor for hyaluronan-mediated motility variant 4 (RHAMMv4), a regulator of MAPKK-MAPK interaction and activation, did not inhibit PDGF-BB-induced p42(MAPK) activation nor did a construct expressing PDGFR-beta with cytoplasmic tyrosines mutated to phenylalanine. However, overexpression of a dominant negative PDGFR-beta lacking the cytoplasmic signaling domain abrogated p42(MAPK) activity. These results suggest that PDGF-BB-mediated activation of p42(MAPK) requires the PDGFR-beta but is independent of its tyrosine phosphorylation.

Diseases/Pathways annotated by Medline MESH: MAP Kinase Signaling System
Document information provided by NCBI PubMed

Text Mining Data

p42(MAPK) → PDGF-BB: " PDGF-BB mediated activation of p42(MAPK) is independent of PDGF beta-receptor tyrosine phosphorylation "

p42(MAPK) → PDGF-BB: " PDGF-BB mediated activation of p42(MAPK) is independent of PDGF beta-receptor tyrosine phosphorylation "

MAPK kinase 2 (MAPKK2) → PDGF-BB: " PDGF-BB also activated MAPK kinase 2 (MAPKK2) but had no effect on MAPKK1 and Raf-1 activity "

p42(MAPK) → PDGF-BB: " Chemical inhibition of Janus kinase, phosphatidylinositol 3-kinase, Src kinase, or tyrosine phosphorylation inhibition of the PDGF beta-receptor ( PDGFR-beta ) did not abrogate PDGF-BB induced p42(MAPK) activation or its threonine or tyrosine phosphorylation "

p42(MAPK) → PDGF-BB: " Chemical inhibition of Janus kinase, phosphatidylinositol 3-kinase, Src kinase, or tyrosine phosphorylation inhibition of the PDGF beta-receptor ( PDGFR-beta ) did not abrogate PDGF-BB induced p42(MAPK) activation or its threonine or tyrosine phosphorylation "

p42(MAPK) → PDGF-BB: " A dominant negative cytoplasmic receptor for hyaluronan mediated motility variant 4 ( RHAMMv4 ), a regulator of MAPKK-MAPK interaction and activation, did not inhibit PDGF-BB induced p42(MAPK) activation nor did a construct expressing PDGFR-beta with cytoplasmic tyrosines mutated to phenylalanine "

p42(MAPK) → PDGF-BB: " A dominant negative cytoplasmic receptor for hyaluronan mediated motility variant 4 ( RHAMMv4 ), a regulator of MAPKK-MAPK interaction and activation, did not inhibit PDGF-BB induced p42(MAPK) activation nor did a construct expressing PDGFR-beta with cytoplasmic tyrosines mutated to phenylalanine "

p42(MAPK) → PDGF-BB: " These results suggest that PDGF-BB mediated activation of p42(MAPK) requires the PDGFR-beta but is independent of its tyrosine phosphorylation "

p42(MAPK) → PDGFR-beta: " These results suggest that PDGF-BB mediated activation of p42(MAPK) requires the PDGFR-beta but is independent of its tyrosine phosphorylation "

p42(MAPK) → PDGF-BB: " These results suggest that PDGF-BB mediated activation of p42(MAPK) requires the PDGFR-beta but is independent of its tyrosine phosphorylation "

p42(MAPK) → PDGFR-beta: " These results suggest that PDGF-BB mediated activation of p42(MAPK) requires the PDGFR-beta but is independent of its tyrosine phosphorylation "

PDGFR-beta → PDGF-BB: " These results suggest that PDGF-BB mediated activation of p42(MAPK) requires the PDGFR-beta but is independent of its tyrosine phosphorylation "

Manually curated Databases

No curated data.