Cancer Lett 2002,
PMID: 12175535
Van Brocklyn, James; Letterle, Catherine; Snyder, Pamela; Prior, Thomas
The regulation of glioma cell proliferation by sphingosine-1-phosphate (S1P) was studied using the human glioblastoma cell line U-373 MG. U-373 MG cells responded mitogenically to nanomolar concentrations of S1P, and express mRNA encoding the S1P receptors S1P1/endothelial differentiation gene (EDG)-1, S1P3/EDG-3 and S1P2/EDG-5. S1P-induced proliferation required extracellular signal-regulated kinase activation and was partially sensitive to pertussis toxin and wortmannin, indicating involvement of a Gi-coupled receptor and phosphatidylinositol 3-kinase. Moreover, S1P1, S1P3 and S1P2 receptors are expressed in the majority of human glioblastomas as determined by reverse transcriptase-polymerase chain reaction analysis. Thus, S1P signaling through EDG receptors may contribute to glioblastoma growth in vivo.
Diseases/Pathways annotated by Medline MESH: Glioma
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Text Mining Data
Dashed line = No text mining data
Manually curated Databases
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NCI Pathway Database S1P1 pathway:
Erk1-2 (MAPK3/MAPK1)
→
S1P1/S1P/Gi complex (S1PR1-GNAI2_GNAI3_GNAO1_GNAO1_GNAZ_GNAI1)
(modification, collaborate)
Evidence: mutant phenotype, assay
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NCI Pathway Database S1P1 pathway:
Erk1-2 (MAPK3/MAPK1)
→
Erk1-2-active (MAPK3/MAPK1)
(modification, collaborate)
Evidence: mutant phenotype, assay
-
NCI Pathway Database S1P1 pathway:
S1P1/S1P/Gi complex (S1PR1-GNAI2_GNAI3_GNAO1_GNAO1_GNAZ_GNAI1)
→
Erk1-2-active (MAPK3/MAPK1)
(modification, activates)
Evidence: mutant phenotype, assay
In total, 20 gene pairs are associated to this article in curated databases