We have a suspicion that you are an automated web bot software, not a real user. To keep our site fast for other users, we have slowed down this page. The slowdown will gradually disappear. If you think this is a mistake, please contact us at genome-www@soe.ucsc.edu. Also note that all data for hgGeneGraph can be obtained through our public MySQL server and all our software source code is available and can be installed locally onto your own computer. If you are unsure how to use these resources, do not hesitate to contact us.
UCSC Genome Browser Gene Interaction Graph
Gene interactions and pathways from curated databases and text-mining
FEBS Lett 2004, PMID: 15527767

Proteomics-based identification of proteins interacting with Smad3: SREBP-2 forms a complex with Smad3 and inhibits its transcriptional activity.

Grimsby, Susanne; Jaensson, Hanna; Dubrovska, Anna; Lomnytska, Marta; Hellman, Ulf; Souchelnytskyi, Serhiy

Smad3 is an important component of transforming growth factor-beta (TGFbeta) intracellular signalling. To identify novel interacting proteins of Smad3, we performed pull-down assays with Smad3 constructs fused to glutathione-S-transferase. Proteins which formed complexes with these constructs were analyzed by two-dimensional gel electrophoresis, and were identified by matrix-assisted laser desorption-ionization time-of-flight mass spectrometry. We identified 14 proteins interacting with the Smad3 construct lacking the N-terminal Mad homology domain 1 (MH1), and 12 proteins interacting with the construct lacking the C-terminal MH2 domain. Proteins involved in signalling processes, in metabolism regulation, novel proteins, and components of cytoskeleton form four groups of interacting proteins. Interactions of AGP7, sex-determining region Y protein, actin beta and sterol regulatory element binding protein-2 (SREBP-2) proteins with Smad3 constructs were confirmed by immunoblotting with specific antibodies. Interaction of Smad3 with SREBP-2 was also confirmed by co-immunoprecipitation of myc-Smad3 and Flag-SREBP-2 upon expression in mammalian cells. We found that SREBP-2 inhibited the transcriptional activity of Smad3 in luciferase reporter assays.

Document information provided by NCBI PubMed

Text Mining Data

Smad3 ⊣ SREBP-2: " We found that SREBP-2 inhibited the transcriptional activity of Smad3 in luciferase reporter assays "

Manually curated Databases

  • IRef Bind Interaction: SMAD3 — SREBF2
  • IRef Bind_translation Interaction: SMAD3 — SREBF2 (affinity chromatography technology)
  • IRef Bind_translation Interaction: SMAD3 — SREBF2 (coimmunoprecipitation)
  • IRef Biogrid Interaction: SRY — SMAD3 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SREBF2 — SMAD3 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: ACTB — SMAD3 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: PRTN3 — SMAD3 (physical association, affinity chromatography technology)
  • IRef Hprd Interaction: PAEP — SMAD3 (in vitro)
  • IRef Hprd Interaction: VIM — SMAD3 (in vitro)
  • IRef Hprd Interaction: EPB41 — SMAD3 (in vitro)
  • IRef Hprd Interaction: IL37 — SMAD3 (in vitro)
  • IRef Hprd Interaction: KRT10 — SMAD3 (in vitro)
  • IRef Hprd Interaction: SRY — SMAD3 (in vitro)
  • IRef Hprd Interaction: SF3A1 — SMAD3 (in vitro)
  • IRef Hprd Interaction: TPM3 — SMAD3 (in vitro)
  • IRef Hprd Interaction: MYO5B — SMAD3 (in vitro)
  • IRef Hprd Interaction: KRT2 — SMAD3 (in vitro)
  • IRef Hprd Interaction: JPH3 — SMAD3 (in vitro)
  • IRef Hprd Interaction: LMNA — SMAD3 (in vitro)
  • IRef Hprd Interaction: ACTB — SMAD3 (in vitro)
  • IRef Hprd Interaction: SMAD3 — GTF2IRD1 (in vitro)
  • IRef Hprd Interaction: PRTN3 — SMAD3 (in vitro)
  • IRef Hprd Interaction: ZDHHC4 — SMAD3 (in vitro)
  • IRef Intact Interaction: Complex of SRY-PAEP-IL36RN-AGL-KRT10-JPH1-SMAD3 (association, pull down)
  • IRef Intact Interaction: SMAD3 — SRY (physical association, pull down)
  • IRef Intact Interaction: SMAD3 — SREBF2 (physical association, pull down)
  • IRef Intact Interaction: SMAD3 — ACTB (physical association, pull down)
  • IRef Intact Interaction: PRTN3 — SMAD3 (physical association, pull down)
In total, 35 gene pairs are associated to this article in curated databases