Gene interactions and pathways from curated databases and text-mining
Journal of proteomics 2011, PMID: 21907836

Interactome study suggests multiple cellular functions of hepatoma-derived growth factor (HDGF).

Zhao, Jian; Yu, Hongxiu; Lin, Ling; Tu, Jun; Cai, Lili; Chen, Yanmei; Zhong, Fan; Lin, Chengzhao; He, Fuchu; Yang, Pengyuan

Hepatoma-derived growth factor (HDGF) is a growth factor related to normal development and tumorigenesis; however, the mechanism of its mitogenic and angiogenic activity still remains unknown. Analysis of the HDGF interactome could be important for understanding its function and integrative mechanisms, because knowledge about HDGF interactors is very limited. In this study, through streptavidin-binding peptide (SBP) and Flag tag-based tandem affinity purification (SBP/Flag-TAP) coupled with LC-MS/MS, 106 proteins were shown to form complexes with HDGF. RNAs were also found in the HDGF complex through the SBP-tag based RNA co-immunoprecipitation (SBP-RIP) assay. Some of these interactions were confirmed by Co-IP and RT-PCR. We then found that the HATH domain was essential for HDGF interactions including protein-protein and protein-RNA interactions, and that in the absence of the HATH domain, NO-HATH could not form complex. The interactome suggests that HDGF is a multifunctional protein and participates in many cellular events, including ribosome biogenesis, RNA processing, DNA damage repair and transcriptional regulation. This new information about the HDGF interactome will further our understanding on HDGF-mediated cellular functions.

Document information provided by NCBI PubMed

Text Mining Data

Dashed line = No text mining data

Manually curated Databases

  • IRef Biogrid Interaction: SRSF1 — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SSRP1 — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: DDX5 — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: HDAC1 — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SRPK1 — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: HDGF — DDX17 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: KRT1 — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SLC25A5 — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: RRP1B — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: HDGF — PHF6 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: HDGF — NACA (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: HDGF — SRSF7 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: HIST1H4A — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: TOP1 — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: HDGF — PGAM5 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: ASUN — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: MPG — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: WDR36 — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: HDGF — DHX30 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: RPL35A — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: HDGF — MRPS27 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: ABCF1 — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: HDGF — GTPBP1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: RPP38 — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: XRCC5 — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: EXOSC2 — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: HDGF — HIST1H1E (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: EEF2 — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: RPS23 — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: U2AF2 — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: HDGF — EFTUD2 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: HDGF — NOP2 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: TSR1 — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: POLRMT — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: BRIX1 — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: RSL1D1 — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: HDGF — HIST1H3B, HIST1H3C, HIST1H3A, HIST1H3F, HIST1H3G, HIST1H3D, HIST1H3E, HIST1H3J, HIST1H3H, HIST1H3I (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: ACTL6A — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: MYBBP1A — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: BAZ1B — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: HDGF — DHX15 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: HDGF — EXOSC3 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: HDGF — SMC3 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: RPS11 — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: DDX21 — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: GNL3 — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: HDGF — H2AFY2 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: RPL35 — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: HDGF — PRSS3 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: EIF2S2 — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: ALYREF — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: TRMT10C — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: RCC1 — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: RPL17 — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: HDGF — PLS3 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: C7orf26 — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: KPNA2 — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: MRTO4 — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: RPS15A — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: RUVBL1 — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: RCL1 — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: EMD — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: PRMT5 — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: HNRNPA2B1 — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: DNAJA1 — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: HIST1H2BD — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: HDGF — MAPK14 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: EXOSC10 — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SSB — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: HDGF — EIF2AK2 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SSR1 — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: HDGF — DEK (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: WDR5 — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: HDGF — HNRNPU (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: RFC3 — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: HDGF — DDX1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: RPA1 — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: INTS10 — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: HDGF — VWA9 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: HNRNPA3 — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: LYAR — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SRSF2 — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: HDGF — RPL19 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: HDGF — HSD17B10 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: RPL22 — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: KRT2 — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: HDGF — STAU1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: MRPS22 — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: HDGF — EBNA1BP2 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: HDGF — MAP4 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: HDGF — RBMX (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SRP72 — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: HP1BP3 — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: C8orf33 — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: POLDIP3 — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: ZC3HAV1 — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: CDC5L — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SMARCA5 — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: HDGF — HSPA9 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: EIF2S3 — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: HDGF — KIF2A (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: RPL32 — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: HDGF — PSIP1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: HDGF — EXOSC6 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: C7orf50 — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: NUDC — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: DIMT1 — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: NCL — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SRSF3 — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SNRPD1 — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: HDGF — SRSF5 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: PRKDC — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: HDGF — RFC4 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: EIF5B — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: HDGF — RFC1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: HDAC2 — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: HIST2H2BE — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: ZRANB2 — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: HDGF — GRWD1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: PARP1 — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: HIST1H1D — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: CBX3 — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: HDGF — U2AF1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: H1FX — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: DHX9 — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: HDGF — KRT10 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: HDGF — TMPO (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: HDGF — RCC2 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: HMGN1 — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: HDGF — RAN (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: DDX47 — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: CKAP4 — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: THOC3 — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: HDGF — ATAD3A (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: FARSB — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: PWP2 — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: XRCC6 — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: HDGF — HMGA2 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: HDGF — SLC25A3 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: RPL34 — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SRP68 — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: HIST1H1C — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: EIF2S1 — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: MAGEB2 — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: BMS1 — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: HDGF — CDK1 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: HDGF — RFC5 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: HDGF — BUB3 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: HDGF — UBA52 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: HDGF — H2AFY (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: FBL — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: LRRC59 — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: DRG1 — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: RBM39 — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: DDX50 — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: HDGF — RPL10 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SUPT16H — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: H1F0 — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: HIST1H2BK — HDGF (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: HDGF — RBBP4 (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: SUB1 — HDGF (physical association, affinity chromatography technology)
  • IRef Intact Interaction: SRSF1 — HDGF (colocalization, fluorescence microscopy)
  • IRef Intact Interaction: HDGF — SSRP1 (colocalization, fluorescence microscopy)
  • IRef Intact Interaction: Complex of 117 proteins (association, tandem affinity purification)
  • IRef Intact Interaction: HDGF — DDX5 (colocalization, fluorescence microscopy)
  • IRef Intact Interaction: Complex of PRKDC-DDX5-SSRP1-PARP1-HDAC1-HDGF-SRSF1 (association, pull down)
  • IRef Intact Interaction: HDGF — HDAC1 (colocalization, fluorescence microscopy)
  • IRef Intact Interaction: Complex of 60 proteins (association, tandem affinity purification)
In total, 6890 gene pairs are associated to this article in curated databases