Gene interactions and pathways from curated databases and text-mining
J Biol Chem 1998, PMID: 9822659

Characterization of cDNAs encoding the p44 and p35 subunits of human translation initiation factor eIF3.

Block, K L; Vornlocher, H P; Hershey, J W

Eukaryotic translation initiation factor 3 (eIF3) is a large multisubunit complex that plays a central role in the initiation of translation. It binds to 40 S ribosomal subunits resulting in dissociation of 80 S ribosomes, stabilizes initiator methionyl-tRNA binding to 40 S subunits, and is required for mRNA binding. eIF3 has an aggregate molecular mass of approximately 600 kDa and comprises at least 10 subunits. The cDNAs encoding eight of the subunits have been cloned previously (p170, p116, p110, p66, p48, p47, p40, and p36). Here we report the cloning and characterization of human cDNAs encoding two more subunits of human eIF3, namely eIF3-p44 and eIF3-p35. These proteins are immunoprecipitated by affinity-purified anti-eIF3-p170 antibodies, indicating they are components of the eIF3 complex. Far Western analysis shows that eIF3-p44 interacts strongly and specifically with the eIF3-p170 subunit, and weakly with p116/p110, p66, p40, and itself. eIF3-p44 contains an RNA recognition motif near its C terminus. Northwestern blotting shows that eIF3-p44 binds 18 S rRNA and beta-globin mRNA. Possession of cloned cDNAs encoding all 10 subunits of eIF3 provides the tools necessary to elucidate the functions of the individual subunits and the structure of the eIF3 complex.

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Text Mining Data

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Manually curated Databases

  • IRef Biogrid Interaction: EIF3G — EIF3D (direct interaction, far western blotting)
  • IRef Biogrid Interaction: EIF3A — EIF3D (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: EIF3C — EIF3G (direct interaction, far western blotting)
  • IRef Biogrid Interaction: EIF3G — EIF3G (direct interaction, far western blotting)
  • IRef Biogrid Interaction: EIF3A — EIF3F (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: EIF3G — EIF3H (direct interaction, far western blotting)
  • IRef Biogrid Interaction: EIF3A — EIF3B (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: EIF3A — EIF3H (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: EIF3A — EIF3J (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: EIF3A — EIF3G (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: EIF3A — EIF3G (direct interaction, far western blotting)
  • IRef Biogrid Interaction: EIF3C — EIF3A (physical association, affinity chromatography technology)
  • IRef Biogrid Interaction: EIF3B — EIF3G (direct interaction, far western blotting)
  • IRef Hprd Interaction: EIF3C — EIF3J (in vivo)
  • IRef Hprd Interaction: Complex of 201 proteins (in vivo)
  • IRef Hprd Interaction: EIF3G — EIF3D (in vivo)
  • IRef Hprd Interaction: EIF3G — EIF3D (in vitro)
  • IRef Hprd Interaction: SIAH1 — EIF3J (in vivo)
  • IRef Hprd Interaction: EIF3C — EIF3G (in vitro)
  • IRef Hprd Interaction: EIF3C — EIF3G (in vivo)
  • IRef Hprd Interaction: EIF3G — EIF3G (in vivo)
  • IRef Hprd Interaction: EIF3G — EIF3G (in vitro)
  • IRef Hprd Interaction: EIF3G — EIF3H (in vivo)
  • IRef Hprd Interaction: EIF3G — EIF3H (in vitro)
  • IRef Hprd Interaction: EIF3A — EIF3B (in vivo)
  • IRef Hprd Interaction: EIF3A — EIF3B (in vitro)
  • IRef Hprd Interaction: EIF3A — EIF3J (in vitro)
  • IRef Hprd Interaction: EIF3A — EIF3J (in vivo)
  • IRef Hprd Interaction: EIF3J — EIF3D (in vivo)
  • IRef Hprd Interaction: EIF3A — EIF3G (in vitro)
  • IRef Hprd Interaction: EIF3A — EIF3G (in vivo)
  • IRef Hprd Interaction: EIF3J — EIF3J (in vivo)
  • IRef Ophid Interaction: EIF3C — EIF3J (aggregation, confirmational text mining)
  • IRef Ophid Interaction: EIF3C — EIF3G (aggregation, confirmational text mining)
  • IRef Ophid Interaction: EIF3G — EIF3H (aggregation, confirmational text mining)
In total, 48 gene pairs are associated to this article in curated databases