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CYP2E1 — INS

Text-mined interactions from Literome

Woodcroft et al., J Pharmacol Exp Ther 1999 : Insulin differentially affects xenobiotic enhanced, cytochrome P-450 (CYP)2E1 , CYP2B, CYP3A, and CYP4A expression in primary cultured rat hepatocytes ... In the present study, we examined the effects of insulin on pyridine-, phenobarbital-, and ciprofibrate mediated expression of CYP2E1 , CYP2B, CYP3A, and CYP4A in primary cultured rat hepatocytes ... To examine whether insulin effects on xenobiotic mediated CYP2E1 expression were selective, insulin effects on xenobiotic mediated expression of transcriptionally regulated CYP2B, CYP3A, and CYP4A were examined ... The results of this study demonstrate that decreasing the insulin level in the primary hepatocyte culture medium enhanced xenobiotic mediated CYP2E1 expression, whereas lower insulin levels suppressed xenobiotic mediated CYP2B, CYP3A, and CYP4A expression in this cell culture system
Son et al., Chem Biol Interact 2000 (Starvation) : The hepatic CYP2E1 induction in hypophysectomized diabetic rats was inhibited by hGH treatment, indicating that the hGH effect on CYP2E1 expression did not involve insulin production
Chitturi et al., Semin Liver Dis 2001 (Chronic Disease...) : CYP2E1 is normally suppressed by insulin but is invariably increased in the livers of patients with NASH
Sidhu et al., Mol Pharmacol 2001 : Recent inhibitor based studies have implicated a mechanistic role for phosphatidylinositol 3' kinase (PI3K) in the insulin mediated suppression of CYP2E1 mRNA levels in hepatocytes ... We investigated the dose dependence for this response and for the effects of insulin and extracellular matrix on PI3K signaling and CYP2E1 mRNA expression levels using a highly defined rat primary hepatocyte culture system
Yang et al., Biosci Biotechnol Biochem 2001 : The effects of insulin on CYP2E1 expression were also measured
Woodcroft et al., Hepatology 2002 : Primary cultured rat hepatocytes were used to explore ketone body and insulin regulation of CYP2E1 expression ... The phosphatidylinositol 3-kinase ( PI3-kinase ) inhibitors wortmannin or LY294002 and rapamycin, an inhibitor of p70 S6 kinase phosphorylation, ameliorated the insulin mediated decrease in CYP2E1 mRNA levels ... Geldanamycin, which inhibits Src kinase, also abrogated the insulin mediated decrease in CYP2E1 mRNA levels ... In contrast, the protein kinase C ( PKC ) inhibitor bisindolylmaleimide, the mitogen activated protein kinase kinase ( MEK ) inhibitor PD98059, and the p38 mitogen activated protein ( MAP ) kinase inhibitor SB202190 did not affect the insulin mediated decrease in CYP2E1 ... Analysis of CYP2E1 gene transcription using heterogeneous nuclear RNA ( hnRNA ) showed that insulin suppressed CYP2E1 transcription ... In conclusion, these data show involvement of transcriptional and posttranscriptional mechanisms in the insulin mediated regulation of CYP2E1 and implicate PI3-kinase, p70 S6 kinase, and Src kinase in mediating these effects
Murdock et al., Biochem Pharmacol 2004 : Role of CYP2E1 in ketone stimulated insulin release in pancreatic B-cells ... The role of CYP2E1 in ketone stimulated insulin release was investigated using isolated pancreatic islets of Langerhans and two mammalian insulin secreting pancreatic beta-cell lines engineered to stably express human CYP2E1 ( designated BRIN BD11h2E1 and INS-1h2E1 )
Schattenberg et al., J Biol Chem 2005 (Fatty Liver...) : Hepatocyte CYP2E1 overexpression and steatohepatitis lead to impaired hepatic insulin signaling ... CYP2E1 overexpression in a hepatocyte cell line decreased tyrosine phosphorylation of insulin receptor substrate (IRS)-1 and IRS-2 in response to insulin