Human methylome studies SRP337018 Track Settings
 
whole-genome wide DNA methylation aberration in HCC [Liver]

Track collection: Human methylome studies

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SRX12151753 
SRX12151754 
SRX12151756 
SRX12151766 
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 SRX12151753  CpG methylation  Liver / SRX12151753 (CpG methylation)   Data format 
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 SRX12151754  CpG methylation  Liver / SRX12151754 (CpG methylation)   Data format 
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 SRX12151756  CpG methylation  Liver / SRX12151756 (CpG methylation)   Data format 
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 SRX12151766  CpG methylation  Liver / SRX12151766 (CpG methylation)   Data format 
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 SRX12151777  CpG methylation  Liver / SRX12151777 (CpG methylation)   Data format 
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 SRX12151788  CpG methylation  Liver / SRX12151788 (CpG methylation)   Data format 
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 SRX12151791  CpG methylation  Liver / SRX12151791 (CpG methylation)   Data format 
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 SRX12151792  CpG methylation  Liver / SRX12151792 (CpG methylation)   Data format 
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 SRX12151793  CpG methylation  Liver / SRX12151793 (CpG methylation)   Data format 
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 SRX12151794  HMR  Liver / SRX12151794 (HMR)   Data format 
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 SRX12151794  CpG methylation  Liver / SRX12151794 (CpG methylation)   Data format 
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 SRX12151795  CpG methylation  Liver / SRX12151795 (CpG methylation)   Data format 
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 SRX12151797  CpG methylation  Liver / SRX12151797 (CpG methylation)   Data format 
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 SRX12151798  CpG methylation  Liver / SRX12151798 (CpG methylation)   Data format 
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 SRX12151799  CpG methylation  Liver / SRX12151799 (CpG methylation)   Data format 
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 SRX12151800  CpG methylation  Liver / SRX12151800 (CpG methylation)   Data format 
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 SRX12151802  CpG methylation  Liver / SRX12151802 (CpG methylation)   Data format 
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 SRX12151803  CpG methylation  Liver / SRX12151803 (CpG methylation)   Data format 
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 SRX12151804  CpG methylation  Liver / SRX12151804 (CpG methylation)   Data format 
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 SRX12151805  CpG methylation  Liver / SRX12151805 (CpG methylation)   Data format 
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 SRX12151806  CpG methylation  Liver / SRX12151806 (CpG methylation)   Data format 
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 SRX12151807  CpG methylation  Liver / SRX12151807 (CpG methylation)   Data format 
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 SRX12151808  CpG methylation  Liver / SRX12151808 (CpG methylation)   Data format 
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 SRX12151809  CpG methylation  Liver / SRX12151809 (CpG methylation)   Data format 
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 SRX12151810  CpG methylation  Liver / SRX12151810 (CpG methylation)   Data format 
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 SRX12151811  HMR  Liver / SRX12151811 (HMR)   Data format 
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 SRX12151811  CpG methylation  Liver / SRX12151811 (CpG methylation)   Data format 
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 SRX12151812  CpG methylation  Liver / SRX12151812 (CpG methylation)   Data format 
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 SRX12151813  CpG methylation  Liver / SRX12151813 (CpG methylation)   Data format 
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 SRX12151814  HMR  Liver / SRX12151814 (HMR)   Data format 
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 SRX12151814  CpG methylation  Liver / SRX12151814 (CpG methylation)   Data format 
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 SRX12151815  HMR  Liver / SRX12151815 (HMR)   Data format 
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 SRX12151815  CpG methylation  Liver / SRX12151815 (CpG methylation)   Data format 
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 SRX12151816  HMR  Liver / SRX12151816 (HMR)   Data format 
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 SRX12151816  CpG methylation  Liver / SRX12151816 (CpG methylation)   Data format 
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 SRX12151817  HMR  Liver / SRX12151817 (HMR)   Data format 
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 SRX12151817  CpG methylation  Liver / SRX12151817 (CpG methylation)   Data format 
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 SRX12151818  HMR  Liver / SRX12151818 (HMR)   Data format 
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 SRX12151818  CpG methylation  Liver / SRX12151818 (CpG methylation)   Data format 
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 SRX12151819  HMR  Liver / SRX12151819 (HMR)   Data format 
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 SRX12151819  CpG methylation  Liver / SRX12151819 (CpG methylation)   Data format 
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 SRX12151821  HMR  Liver / SRX12151821 (HMR)   Data format 
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 SRX12151821  CpG methylation  Liver / SRX12151821 (CpG methylation)   Data format 
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 SRX12151822  HMR  Liver / SRX12151822 (HMR)   Data format 
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 SRX12151822  CpG methylation  Liver / SRX12151822 (CpG methylation)   Data format 
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 SRX12151823  HMR  Liver / SRX12151823 (HMR)   Data format 
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 SRX12151823  CpG methylation  Liver / SRX12151823 (CpG methylation)   Data format 
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 SRX12151824  HMR  Liver / SRX12151824 (HMR)   Data format 
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 SRX12151824  CpG methylation  Liver / SRX12151824 (CpG methylation)   Data format 
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 SRX12151825  HMR  Liver / SRX12151825 (HMR)   Data format 
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 SRX12151825  CpG methylation  Liver / SRX12151825 (CpG methylation)   Data format 
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 SRX12151826  HMR  Liver / SRX12151826 (HMR)   Data format 
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 SRX12151826  CpG methylation  Liver / SRX12151826 (CpG methylation)   Data format 
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 SRX12151828  HMR  Liver / SRX12151828 (HMR)   Data format 
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 SRX12151828  CpG methylation  Liver / SRX12151828 (CpG methylation)   Data format 
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 SRX12151829  HMR  Liver / SRX12151829 (HMR)   Data format 
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 SRX12151829  CpG methylation  Liver / SRX12151829 (CpG methylation)   Data format 
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 SRX12151830  HMR  Liver / SRX12151830 (HMR)   Data format 
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 SRX12151830  CpG methylation  Liver / SRX12151830 (CpG methylation)   Data format 
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 SRX12151831  HMR  Liver / SRX12151831 (HMR)   Data format 
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 SRX12151831  CpG methylation  Liver / SRX12151831 (CpG methylation)   Data format 
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 SRX12151832  HMR  Liver / SRX12151832 (HMR)   Data format 
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 SRX12151832  CpG methylation  Liver / SRX12151832 (CpG methylation)   Data format 
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 SRX12151833  HMR  Liver / SRX12151833 (HMR)   Data format 
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 SRX12151833  CpG methylation  Liver / SRX12151833 (CpG methylation)   Data format 
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 SRX12151834  CpG methylation  Liver / SRX12151834 (CpG methylation)   Data format 
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 SRX12151835  HMR  Liver / SRX12151835 (HMR)   Data format 
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 SRX12151835  CpG methylation  Liver / SRX12151835 (CpG methylation)   Data format 
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 SRX12151836  HMR  Liver / SRX12151836 (HMR)   Data format 
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 SRX12151836  CpG methylation  Liver / SRX12151836 (CpG methylation)   Data format 
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 SRX12151837  HMR  Liver / SRX12151837 (HMR)   Data format 
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 SRX12151837  CpG methylation  Liver / SRX12151837 (CpG methylation)   Data format 
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 SRX12151838  HMR  Liver / SRX12151838 (HMR)   Data format 
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 SRX12151838  CpG methylation  Liver / SRX12151838 (CpG methylation)   Data format 
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 SRX12151839  CpG methylation  Liver / SRX12151839 (CpG methylation)   Data format 
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 SRX12151840  HMR  Liver / SRX12151840 (HMR)   Data format 
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 SRX12151840  CpG methylation  Liver / SRX12151840 (CpG methylation)   Data format 
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 SRX12151841  HMR  Liver / SRX12151841 (HMR)   Data format 
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 SRX12151841  CpG methylation  Liver / SRX12151841 (CpG methylation)   Data format 
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 SRX12151842  HMR  Liver / SRX12151842 (HMR)   Data format 
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 SRX12151842  CpG methylation  Liver / SRX12151842 (CpG methylation)   Data format 
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 SRX12151843  HMR  Liver / SRX12151843 (HMR)   Data format 
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 SRX12151843  CpG methylation  Liver / SRX12151843 (CpG methylation)   Data format 
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 SRX12151844  HMR  Liver / SRX12151844 (HMR)   Data format 
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 SRX12151844  CpG methylation  Liver / SRX12151844 (CpG methylation)   Data format 
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 SRX12151845  CpG methylation  Liver / SRX12151845 (CpG methylation)   Data format 
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 SRX12151846  HMR  Liver / SRX12151846 (HMR)   Data format 
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 SRX12151846  CpG methylation  Liver / SRX12151846 (CpG methylation)   Data format 
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 SRX12151847  HMR  Liver / SRX12151847 (HMR)   Data format 
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 SRX12151847  CpG methylation  Liver / SRX12151847 (CpG methylation)   Data format 
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 SRX12151848  HMR  Liver / SRX12151848 (HMR)   Data format 
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 SRX12151848  CpG methylation  Liver / SRX12151848 (CpG methylation)   Data format 
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 SRX12151849  HMR  Liver / SRX12151849 (HMR)   Data format 
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 SRX12151849  CpG methylation  Liver / SRX12151849 (CpG methylation)   Data format 
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 SRX12151851  HMR  Liver / SRX12151851 (HMR)   Data format 
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 SRX12151851  CpG methylation  Liver / SRX12151851 (CpG methylation)   Data format 
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 SRX12151852  HMR  Liver / SRX12151852 (HMR)   Data format 
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 SRX12151852  CpG methylation  Liver / SRX12151852 (CpG methylation)   Data format 
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 SRX12151854  CpG methylation  Liver / SRX12151854 (CpG methylation)   Data format 
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 SRX12151863  CpG methylation  Liver / SRX12151863 (CpG methylation)   Data format 
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 SRX12151874  CpG methylation  Liver / SRX12151874 (CpG methylation)   Data format 
    
Assembly: Human Dec. 2013 (GRCh38/hg38)

Study title: whole-genome wide DNA methylation aberration in HCC
SRA: SRP337018
GEO: not found
Pubmed: not found

Experiment Label Methylation Coverage HMRs HMR size AMRs AMR size PMDs PMD size Conversion Title
SRX12151753 Liver 0.582 15.1 64909 13526.4 669 910.1 4024 290224.2 0.974 WGBS of Homo Sapiens:tumor tissues of HCC
SRX12151754 Liver 0.537 15.0 52435 18858.0 5800 5725.0 5565 219044.1 0.973 WGBS of Homo Sapiens:tumor tissues of HCC
SRX12151756 Liver 0.678 13.0 47416 17189.1 12585 5609.9 2353 524993.7 0.975 WGBS of Homo Sapiens:tumor tissues of HCC
SRX12151766 Liver 0.563 15.4 68111 15523.0 4480 13793.6 6666 174601.6 0.974 WGBS of Homo Sapiens:tumor tissues of HCC
SRX12151777 Liver 0.583 16.7 19199 33714.0 33933 1958.4 3113 511318.1 0.963 WGBS of Homo Sapiens:tumor tissues of HCC
SRX12151788 Liver 0.703 16.9 78008 7187.8 1017 1055.7 2939 317324.5 0.977 WGBS of Homo Sapiens:tumor tissues of HCC
SRX12151791 Liver 0.497 16.2 60789 19503.0 5454 5793.4 8056 166450.1 0.975 WGBS of Homo Sapiens:tumor tissues of HCC
SRX12151792 Liver 0.711 15.4 71582 5854.5 293 1181.3 4395 145454.9 0.975 WGBS of Homo Sapiens:tumor tissues of HCC
SRX12151793 Liver 0.381 15.0 100556 14639.3 759 1066.0 8721 178974.2 0.973 WGBS of Homo Sapiens:tumor tissues of HCC
SRX12151794 Liver 0.759 15.2 48213 2446.6 1163 969.7 2043 461695.6 0.976 WGBS of Homo Sapiens:tumor tissues of HCC
SRX12151795 Liver 0.490 13.1 58076 21778.4 4252 14411.1 5768 274565.6 0.974 WGBS of Homo Sapiens:tumor tissues of HCC
SRX12151797 Liver 0.637 14.9 59582 11472.1 1877 958.5 3851 235902.8 0.976 WGBS of Homo Sapiens:tumor tissues of HCC
SRX12151798 Liver 0.538 14.6 75107 14581.1 1733 11328.6 4762 239748.0 0.974 WGBS of Homo Sapiens:tumor tissues of HCC
SRX12151799 Liver 0.612 13.9 46134 18090.3 2451 929.6 3409 400055.7 0.977 WGBS of Homo Sapiens:tumor tissues of HCC
SRX12151800 Liver 0.698 14.5 61959 9237.6 256 931.1 2539 395033.6 0.971 WGBS of Homo Sapiens:tumor tissues of HCC
SRX12151802 Liver 0.518 14.2 75196 15213.7 524 882.5 5760 229644.5 0.972 WGBS of Homo Sapiens:tumor tissues of HCC
SRX12151803 Liver 0.423 14.2 52774 24311.4 1859 15554.6 6718 227221.8 0.968 WGBS of Homo Sapiens:tumor tissues of HCC
SRX12151804 Liver 0.642 17.6 64458 10880.1 2111 993.6 4154 187690.5 0.962 WGBS of Homo Sapiens:tumor tissues of HCC
SRX12151805 Liver 0.492 15.7 82022 15469.3 3675 5885.6 6818 214341.0 0.953 WGBS of Homo Sapiens:tumor tissues of HCC
SRX12151806 Liver 0.730 16.2 72021 6907.0 2351 924.2 3582 209058.0 0.960 WGBS of Homo Sapiens:tumor tissues of HCC
SRX12151807 Liver 0.430 15.3 92901 15024.1 1476 1153.4 7823 187750.5 0.965 WGBS of Homo Sapiens:tumor tissues of HCC
SRX12151808 Liver 0.671 15.7 55614 13846.2 4685 991.9 3550 319291.9 0.954 WGBS of Homo Sapiens:tumor tissues of HCC
SRX12151809 Liver 0.729 16.4 59388 9984.5 2517 1017.5 2252 457159.2 0.941 WGBS of Homo Sapiens:tumor tissues of HCC
SRX12151810 Liver 0.494 13.0 59843 19039.7 2737 7531.7 5396 255920.1 0.975 WGBS of Homo Sapiens:tumor tissues of HCC
SRX12151811 Liver 0.696 17.1 35214 1321.1 723 900.1 2962 44478.2 0.978 WGBS of Homo Sapiens:tumor tissues of HCC
SRX12151812 Liver 0.539 16.0 43818 23329.9 14391 3015.1 4285 338650.7 0.976 WGBS of Homo Sapiens:tumor tissues of HCC
SRX12151813 Liver 0.445 16.9 91919 13601.1 1809 1072.8 7891 166532.2 0.970 WGBS of Homo Sapiens:tumor tissues of HCC
SRX12151814 Liver 0.741 15.3 34738 1447.0 1501 925.7 2500 383060.9 0.971 WGBS of Homo Sapiens:non-tumor tissues of HCC
SRX12151815 Liver 0.737 13.0 45711 2302.0 612 893.0 2132 526602.7 0.970 WGBS of Homo Sapiens:non-tumor tissues of HCC
SRX12151816 Liver 0.733 13.4 36245 1591.6 1369 944.6 1915 560909.5 0.972 WGBS of Homo Sapiens:non-tumor tissues of HCC
SRX12151817 Liver 0.741 14.6 33411 1411.5 1228 924.5 2489 397803.1 0.972 WGBS of Homo Sapiens:non-tumor tissues of HCC
SRX12151818 Liver 0.741 14.8 33568 1445.2 838 902.9 2426 407390.6 0.973 WGBS of Homo Sapiens:non-tumor tissues of HCC
SRX12151819 Liver 0.742 13.6 36194 1463.3 1228 903.6 3002 297624.6 0.977 WGBS of Homo Sapiens:non-tumor tissues of HCC
SRX12151821 Liver 0.737 13.6 44018 1995.1 769 923.4 1976 552398.4 0.977 WGBS of Homo Sapiens:non-tumor tissues of HCC
SRX12151822 Liver 0.788 13.4 61162 2928.8 1976 1221.2 3750 129286.1 0.974 WGBS of Homo Sapiens:tumor tissues of HCC
SRX12151823 Liver 0.758 14.3 39324 1413.6 1248 909.8 3980 193726.7 0.978 WGBS of Homo Sapiens:non-tumor tissues of HCC
SRX12151824 Liver 0.757 16.9 33737 1289.4 1536 941.6 2491 13487.2 0.979 WGBS of Homo Sapiens:non-tumor tissues of HCC
SRX12151825 Liver 0.755 14.9 33432 1376.1 656 877.6 1802 17391.2 0.972 WGBS of Homo Sapiens:non-tumor tissues of HCC
SRX12151826 Liver 0.767 13.5 37239 1366.7 973 890.9 2683 18276.5 0.976 WGBS of Homo Sapiens:non-tumor tissues of HCC
SRX12151828 Liver 0.745 15.1 47459 1959.5 792 892.5 2139 467445.7 0.972 WGBS of Homo Sapiens:non-tumor tissues of HCC
SRX12151829 Liver 0.767 16.5 42204 1528.2 2381 959.8 5145 112404.3 0.952 WGBS of Homo Sapiens:non-tumor tissues of HCC
SRX12151830 Liver 0.742 14.6 34685 1414.9 1071 878.3 3324 266959.6 0.968 WGBS of Homo Sapiens:non-tumor tissues of HCC
SRX12151831 Liver 0.756 15.8 34997 1351.9 1788 924.7 3655 24644.3 0.974 WGBS of Homo Sapiens:non-tumor tissues of HCC
SRX12151832 Liver 0.728 15.0 44831 1931.1 1435 943.2 2156 514330.0 0.977 WGBS of Homo Sapiens:non-tumor tissues of HCC
SRX12151833 Liver 0.733 14.2 37267 1567.4 1419 927.9 2044 529226.7 0.976 WGBS of Homo Sapiens:non-tumor tissues of HCC
SRX12151834 Liver 0.731 16.3 72857 6144.0 3999 1054.5 2594 322415.0 0.980 WGBS of Homo Sapiens:tumor tissues of HCC
SRX12151835 Liver 0.733 12.9 34111 1359.2 949 918.0 5649 28015.4 0.977 WGBS of Homo Sapiens:non-tumor tissues of HCC
SRX12151836 Liver 0.749 15.4 37979 1363.8 2268 957.5 6718 39488.4 0.977 WGBS of Homo Sapiens:non-tumor tissues of HCC
SRX12151837 Liver 0.764 12.6 40778 1685.0 480 895.3 2262 419526.8 0.975 WGBS of Homo Sapiens:non-tumor tissues of HCC
SRX12151838 Liver 0.764 14.9 45014 1458.4 652 924.9 5064 124728.1 0.974 WGBS of Homo Sapiens:non-tumor tissues of HCC
SRX12151839 Liver 0.698 12.7 64516 7279.5 389 862.3 2303 501863.8 0.975 WGBS of Homo Sapiens:non-tumor tissues of HCC
SRX12151840 Liver 0.749 12.9 32603 1366.3 734 898.5 1761 16896.7 0.972 WGBS of Homo Sapiens:non-tumor tissues of HCC
SRX12151841 Liver 0.747 13.9 37690 1485.0 944 922.4 2150 469817.5 0.972 WGBS of Homo Sapiens:non-tumor tissues of HCC
SRX12151842 Liver 0.756 14.4 42839 1984.0 877 938.4 1967 526562.8 0.956 WGBS of Homo Sapiens:non-tumor tissues of HCC
SRX12151843 Liver 0.753 18.6 48388 1899.2 3141 967.4 2272 424962.5 0.957 WGBS of Homo Sapiens:non-tumor tissues of HCC
SRX12151844 Liver 0.749 16.8 47133 2053.7 1584 956.4 2045 525841.8 0.957 WGBS of Homo Sapiens:non-tumor tissues of HCC
SRX12151845 Liver 0.654 13.4 57425 14189.9 767 996.6 3398 307470.1 0.976 WGBS of Homo Sapiens:tumor tissues of HCC
SRX12151846 Liver 0.765 16.2 46323 2075.1 2153 964.2 2119 459656.0 0.956 WGBS of Homo Sapiens:non-tumor tissues of HCC
SRX12151847 Liver 0.759 15.6 40811 1880.1 2178 956.6 2113 476553.8 0.957 WGBS of Homo Sapiens:non-tumor tissues of HCC
SRX12151848 Liver 0.748 15.6 48396 2658.5 1501 938.8 2012 533504.3 0.957 WGBS of Homo Sapiens:non-tumor tissues of HCC
SRX12151849 Liver 0.763 16.5 41562 1431.7 1619 915.6 6519 65982.8 0.974 WGBS of Homo Sapiens:non-tumor tissues of HCC
SRX12151851 Liver 0.732 16.7 40518 1651.7 1829 924.7 2099 519830.2 0.975 WGBS of Homo Sapiens:non-tumor tissues of HCC
SRX12151852 Liver 0.734 16.9 42702 1778.6 1342 989.1 2247 430900.8 0.968 WGBS of Homo Sapiens:non-tumor tissues of HCC
SRX12151854 Liver 0.500 15.8 44485 23869.4 8488 4275.0 5465 256488.1 0.977 WGBS of Homo Sapiens:tumor tissues of HCC
SRX12151863 Liver 0.598 13.6 73212 12220.0 1516 1019.4 6269 171515.9 0.976 WGBS of Homo Sapiens:tumor tissues of HCC
SRX12151874 Liver 0.547 14.5 46940 19838.2 9948 3841.8 5810 222097.4 0.976 WGBS of Homo Sapiens:tumor tissues of HCC

Methods

All analysis was done using a bisulfite sequnecing data analysis pipeline DNMTools developed in the Smith lab at USC.

Mapping reads from bisulfite sequencing: Bisulfite treated reads are mapped to the genomes with the abismal program. Input reads are filtered by their quality, and adapter sequences in the 3' end of reads are trimmed. This is done with cutadapt. Uniquely mapped reads with mismatches/indels below given threshold are retained. For pair-end reads, if the two mates overlap, the overlapping part of the mate with lower quality is discarded. After mapping, we use the format command in dnmtools to merge mates for paired-end reads. We use the dnmtools uniq command to randomly select one from multiple reads mapped exactly to the same location. Without random oligos as UMIs, this is our best indication of PCR duplicates.

Estimating methylation levels: After reads are mapped and filtered, the dnmtools counts command is used to obtain read coverage and estimate methylation levels at individual cytosine sites. We count the number of methylated reads (those containing a C) and the number of unmethylated reads (those containing a T) at each nucleotide in a mapped read that corresponds to a cytosine in the reference genome. The methylation level of that cytosine is estimated as the ratio of methylated to total reads covering that cytosine. For cytosines in the symmetric CpG sequence context, reads from the both strands are collapsed to give a single estimate. Very rarely do the levels differ between strands (typically only if there has been a substitution, as in a somatic mutation), and this approach gives a better estimate.

Bisulfite conversion rate: The bisulfite conversion rate for an experiment is estimated with the dnmtools bsrate command, which computes the fraction of successfully converted nucleotides in reads (those read out as Ts) among all nucleotides in the reads mapped that map over cytosines in the reference genome. This is done either using a spike-in (e.g., lambda), the mitochondrial DNA, or the nuclear genome. In the latter case, only non-CpG sites are used. While this latter approach can be impacted by non-CpG cytosine methylation, in practice it never amounts to much.

Identifying hypomethylated regions (HMRs): In most mammalian cells, the majority of the genome has high methylation, and regions of low methylation are typically the interesting features. (This seems to be true for essentially all healthy differentiated cell types, but not cells of very early embryogenesis, various germ cells and precursors, and placental lineage cells.) These are valleys of low methylation are called hypomethylated regions (HMR) for historical reasons. To identify the HMRs, we use the dnmtools hmr command, which uses a statistical model that accounts for both the methylation level fluctations and the varying amounts of data available at each CpG site.

Partially methylated domains: Partially methylated domains are large genomic regions showing partial methylation observed in immortalized cell lines and cancerous cells. The pmd program is used to identify PMDs.

Allele-specific methylation: Allele-Specific methylated regions refers to regions where the parental allele is differentially methylated compared to the maternal allele. The program allelic is used to compute allele-specific methylation score can be computed for each CpG site by testing the linkage between methylation status of adjacent reads, and the program amrfinder is used to identify regions with allele-specific methylation.

For more detailed description of the methods of each step, please refer to the DNMTools documentation.