FANTOM5 TSS activity (TPM) Track Settings
 
FANTOM5: TSS activity per sample (TPM)

Track collection: FANTOM5: Mapped transcription start sites (TSS) and their usage

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  Strand: forward reverse
 All Sequence Tech hCAGE  LQhCAGE 
Category
cellLine 
fractionation 
primaryCell 
tissue 
AoSMC response to FGF2 timecourse 
AoSMC response to IL1b timecourse 
ES to cardiomyocyte timecourse 
Embryoid body to melanocyte timecourse 
Epithelial to mesenchymal timecourse 
Human iPS to neuron Downs syndrome 1 timecourse 
Human iPS to neuron Downs syndrome 2 timecourse 
Human iPS to neuron wt 1 timecourse 
Human iPS to neuron wt 2 timecourse 
Lymphatic EC response to VEGFC timecourse 
MCF7 response to EGF timecourse 
MCF7 response to HRG timecourse 
MSC to adipocyte human timecourse 
Macrophage influenza infection timecourse 
Macrophage response to LPS timecourse 
Myoblast to myotube wt and DMD timecourse 
Preadipocyte to adipocyte timecourse 
Rinderpest infection series timecourse 
Saos calcification timecourse 
other samples in timecourse 
Category
 All Sequence Tech hCAGE  LQhCAGE 
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dense
 Configure 		 AoSMC response to FGF2 timecourse  hCAGE  Aortic smooth muscle cell response to FGF2, 00hr00min, biol_rep1 (LK1)_CNhs13339_12642-134G5_forward    Data format 
dense
 Configure 		 AoSMC response to FGF2 timecourse  hCAGE  Aortic smooth muscle cell response to FGF2, 00hr00min, biol_rep1 (LK1)_CNhs13339_12642-134G5_reverse    Data format 
dense
 Configure 		 AoSMC response to FGF2 timecourse  hCAGE  Aortic smooth muscle cell response to FGF2, 00hr00min, biol_rep2 (LK2)_CNhs13358_12740-135I4_forward    Data format 
dense
 Configure 		 AoSMC response to FGF2 timecourse  hCAGE  Aortic smooth muscle cell response to FGF2, 00hr00min, biol_rep2 (LK2)_CNhs13358_12740-135I4_reverse    Data format 
dense
 Configure 		 AoSMC response to IL1b timecourse  hCAGE  Aortic smooth muscle cell response to IL1b, 00hr00min, biol_rep1 (LK31)_CNhs13349_12652-134H6_forward    Data format 
dense
 Configure 		 AoSMC response to IL1b timecourse  hCAGE  Aortic smooth muscle cell response to IL1b, 00hr00min, biol_rep1 (LK31)_CNhs13349_12652-134H6_reverse    Data format 
dense
 Configure 		 AoSMC response to IL1b timecourse  hCAGE  Aortic smooth muscle cell response to IL1b, 00hr00min, biol_rep2 (LK32)_CNhs13369_12750-136A5_forward    Data format 
dense
 Configure 		 AoSMC response to IL1b timecourse  hCAGE  Aortic smooth muscle cell response to IL1b, 00hr00min, biol_rep2 (LK32)_CNhs13369_12750-136A5_reverse    Data format 
dense
 Configure 		 primaryCell  hCAGE  CD14+ Monocytes, donor1_CNhs10852_11224-116B9_forward    Data format 
dense
 Configure 		 primaryCell  hCAGE  CD14+ Monocytes, donor1_CNhs10852_11224-116B9_reverse    Data format 
dense
 Configure 		 primaryCell  hCAGE  CD14+ Monocytes, donor2_CNhs11954_11305-117B9_forward    Data format 
dense
 Configure 		 primaryCell  hCAGE  CD14+ Monocytes, donor2_CNhs11954_11305-117B9_reverse    Data format 
dense
 Configure 		 primaryCell  hCAGE  CD14+ Monocytes, donor3_CNhs11997_11381-118B4_forward    Data format 
dense
 Configure 		 primaryCell  hCAGE  CD14+ Monocytes, donor3_CNhs11997_11381-118B4_reverse    Data format 
dense
 Configure 		 primaryCell  hCAGE  CD4+ T Cells, donor1_CNhs10853_11225-116C1_forward    Data format 
dense
 Configure 		 primaryCell  hCAGE  CD4+ T Cells, donor1_CNhs10853_11225-116C1_reverse    Data format 
dense
 Configure 		 primaryCell  hCAGE  CD4+ T Cells, donor2_CNhs11955_11306-117C1_forward    Data format 
dense
 Configure 		 primaryCell  hCAGE  CD4+ T Cells, donor2_CNhs11955_11306-117C1_reverse    Data format 
dense
 Configure 		 primaryCell  hCAGE  CD4+ T Cells, donor3_CNhs11998_11382-118B5_forward    Data format 
dense
 Configure 		 primaryCell  hCAGE  CD4+ T Cells, donor3_CNhs11998_11382-118B5_reverse    Data format 
    
Source data version: FANTOM5 phase2.5
Assembly: Human Feb. 2009 (GRCh37/hg19)

Description

The FANTOM5 track shows mapped transcription start sites (TSS) and their usage in primary cells, cell lines, and tissues to produce a comprehensive overview of gene expression across the human body by using single molecule sequencing.

Display Conventions and Configuration

Items in this track are colored according to their strand orientation. Blue indicates alignment to the negative strand, and red indicates alignment to the positive strand.

Methods

Protocol

Individual biological states are profiled by HeliScopeCAGE, which is a variation of the CAGE (Cap Analysis Gene Expression) protocol based on a single molecule sequencer. The standard protocol requiring 5 µg of total RNA as a starting material is referred to as hCAGE, and an optimized version for a lower quantity (~ 100 ng) is referred to as LQhCAGE (Kanamori-Katyama et al. 2011).

  • hCAGE
  • LQhCAGE

Samples

Transcription start sites (TSSs) were mapped and their usage in human and mouse primary cells, cell lines, and tissues was to produce a comprehensive overview of mammalian gene expression across the human body. 5′-end of the mapped CAGE reads are counted at a single base pair resolution (CTSS, CAGE tag starting sites) on the genomic coordinates, which represent TSS activities in the sample. Individual samples shown in "TSS activity" tracks are grouped as below.

  • Primary cell
  • Tissue
  • Cell Line
  • Time course
  • Fractionation

TSS peaks and enhancers

TSS (CAGE) peaks across the panel of the biological states (samples) are identified by DPI (decomposition based peak identification, Forrest et al. 2014), where each of the peaks consists of neighboring and related TSSs. The peaks are used as anchors to define promoters and units of promoter-level expression analysis. Two subsets of the peaks are defined based on evidence of read counts, depending on scopes of subsequent analyses, and the first subset (referred as robust set of the peaks, thresholded for expression analysis is shown as TSS peaks. They are named as "p#@GENE_SYMBOL" if associated with 5'-end of known genes, or "p@CHROM:START..END,STRAND" otherwise. The CAGE data is also used to produce an atlas of active, in vivo-transcribed enhancers (Andersson et al. 2014). The summary tracks consist of the TSS (CAGE) peaks, and summary profiles of TSS activities (total and maximum values). The summary track consists of the following tracks.

  • TSS (CAGE) peaks
    • the robust peaks
  • Enhancers
  • TSS summary profiles
    • Total counts and TPM (tags per million) in all the samples
    • Maximum counts and TPM among the samples

TSS activity

5′-end of the mapped CAGE reads are counted at a single base pair resolution (CTSS, CAGE tag starting sites) on the genomic coordinates, which represent TSS activities in the sample. The read counts tracks indicate raw counts of CAGE reads, and the TPM tracks indicate normalized counts as TPM (tags per million).

Categories of individual samples
- Cell Line hCAGE
- Cell Line LQhCAGE
- fractionation hCAGE
- Primary cell hCAGE
- Primary cell LQhCAGE
- Time course hCAGE
- Tissue hCAGE

FANTOM-NET enhancers

A set of enhancers consist of the ones identified by Andersson et al. 2014 and the ones by Hirabayashi et al. 2019

FANTOM CAT

FANTOM CAGE associated transcriptome (FANTOM CAT) is a meta-assembly where FANTOM5 CAGE datasets were integrated with transcript models from diverse sources. Transcription Initiation Evidence Score (TIEScore) is a custom metric that evaluates the properties of a pair of CAGE cluster and transcript model to quantify the likelihood that the corresponding CAGE transcription start site (TSS) is genuine. TIEScore was first applied to each of the five transcript model collections separately and then merged into a non-redundant transcript set. Specifically, the transcript models from GENCODEv19 were used as the initial reference to sequentially overlay onto them the transcripts from the other four collections, in sequence of Human BodyMap 2.0, miTranscriptome, ENCODE, and FANTOM5 RNA-seq assembly. For more details, please refer to Hon et al. 2017.

Data Access

FANTOM5 data can be explored interactively with the Table Browser and cross-referenced with the Data Integrator. For programmatic access, the track can be accessed using the Genome Browser's REST API. ReMap annotations can be downloaded from the Genome Browser's download server as a bigBed file. This compressed binary format can be remotely queried through command line utilities. Please note that some of the download files can be quite large.

The FANTOM5 reprocessed data can be found and downloaded on the FANTOM website.

Credits

Thanks to the FANTOM5 consortium, the Large Scale Data Managing Unit and Preventive Medicine and Applied Genomics Unit, Center for Integrative Medical Sciences (IMS), and RIKEN for providing this data and its analysis.

References

Andersson R, Gebhard C, Miguel-Escalada I, Hoof I, Bornholdt J, Boyd M, Chen Y, Zhao X, Schmidl C, Suzuki T et al. An atlas of active enhancers across human cell types and tissues. Nature. 2014 Mar 27;507(7493):455-461. PMID: 24670763; PMC: PMC5215096

FANTOM Consortium and the RIKEN PMI and CLST (DGT), Forrest AR, Kawaji H, Rehli M, Baillie JK, de Hoon MJ, Haberle V, Lassmann T, Kulakovskiy IV, Lizio M et al. A promoter-level mammalian expression atlas. Nature. 2014 Mar 27;507(7493):462-70. PMID: 24670764; PMC: PMC4529748

Hirabayashi S, Bhagat S, Matsuki Y, Takegami Y, Uehata T, Kanemaru A, Itoh M, Shirakawa K, Takaori- Kondo A, Takeuchi O et al. NET-CAGE characterizes the dynamics and topology of human transcribed cis-regulatory elements. Nat Genet. 2019 Sep;51(9):1369-1379. PMID: 31477927

Hon CC, Ramilowski JA, Harshbarger J, Bertin N, Rackham OJ, Gough J, Denisenko E, Schmeier S, Poulsen TM, Severin J et al. An atlas of human long non-coding RNAs with accurate 5' ends. Nature. 2017 Mar 9;543(7644):199-204. PMID: 28241135; PMC: PMC6857182

Kanamori-Katayama M, Itoh M, Kawaji H, Lassmann T, Katayama S, Kojima M, Bertin N, Kaiho A, Ninomiya N, Daub CO et al. Unamplified cap analysis of gene expression on a single-molecule sequencer. Genome Res. 2011 Jul;21(7):1150-9. PMID: 21596820; PMC: PMC3129257

Lizio M, Harshbarger J, Shimoji H, Severin J, Kasukawa T, Sahin S, Abugessaisa I, Fukuda S, Hori F, Ishikawa-Kato S et al. Gateways to the FANTOM5 promoter level mammalian expression atlas. Genome Biol. 2015 Jan 5;16(1):22. PMID: 25723102; PMC: PMC4310165